Densimetric determination of carbohydrate content in glycoproteins
| Autor: | Tsutomu Arakawa, Keith Langley, Thomas W. Strickland, William C. Kenney, Carl G. Kolvenbach |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Glycosylation
Platelet-derived growth factor Carbohydrates Biophysics CHO Cells Hematopoietic Cell Growth Factors Biochemistry chemistry.chemical_compound Cricetulus Cricetinae Granulocyte Colony-Stimulating Factor Animals Erythropoietin Polyacrylamide gel electrophoresis Glycoproteins Platelet-Derived Growth Factor chemistry.chemical_classification biology Chinese hamster ovary cell Carbohydrate Sialic acid chemistry biology.protein Female Glycoprotein Platelet-derived growth factor receptor Densitometry |
| Zdroj: | Journal of Biochemical and Biophysical Methods. 23:295-300 |
| ISSN: | 0165-022X |
| Popis: | Carbohydrates play important roles in activity, stability and pharmacokinetics of glycoproteins and the degree of glycosylation varies with proteins. In this communication, a simple method of determining the carbohydrate content was developed, which consists of measuring the density increments of a glycoprotein and its non-glycosylated counterpart, and then dividing the difference between the two values by the density increment of carbohydrates. The density increment was relatively constant for various sugars except for sialic acid, and hence assumed to be 0.39. Thus, we obtained carbohydrate contents of 38, 28, 8 and 7% for Chinese hamster ovary cell-expressed erythropoietin (EPO), stem cell factor (SCF), granulocyte-colony-stimulating factor (G-CSF), and platelet-derived growth factor (PDGF), respectively. These values are in close agreement with those determined by other methods. |
| Databáze: | OpenAIRE |
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