The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows
| Autor: | Jacek Wawrzykowski, Monika Jamioł, Marta Kankofer, Wioleta Mojsym |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cows Placenta Antioxidants Superoxide dismutase Andrology Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Fetus Pregnancy Blood plasma Genetics medicine Animals Molecular Biology chemistry.chemical_classification Glutathione Peroxidase 030219 obstetrics & reproductive medicine biology Superoxide Dismutase Glutathione peroxidase Placentation General Medicine medicine.disease Oxidants 030104 developmental biology medicine.anatomical_structure chemistry Oxidative stress biology.protein Cattle Female Original Article Lipid Peroxidation |
| Zdroj: | Molecular Biology Reports |
| ISSN: | 1573-4978 |
| Popis: | Physiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- and antioxidative parameters during placentation phase in bovine placenta is maintained. Placental and blood samples were collected from healthy, HF, pregnant (2nd-3rd month) cows (n = 8) in slaughterhouse and in farm, respectively. Formylokinurenine and bityrosine content were measured spectrofluorimetrically in blood plasma and tissue homogenates while metabolites of lipid peroxidation, total antioxidant capacity, SH groups and activity of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) were determined in examined tissues by spectrophotometry. Western blotting was used to confirm the presence of enzymatic proteins in placenta. Results: Local profile in tissues was more pronounced than general profile in blood plasma. Activities of antioxidative enzymes were significantly (p |
| Databáze: | OpenAIRE |
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