Structure and function of SV40 large-T antigen
| Autor: | David P. Lane, Sara E. Mole, M J Ford, J V Gannon |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
DNA Replication
Antigens Polyomavirus Transforming viruses DNA replication Antibodies Monoclonal Enzyme-Linked Immunosorbent Assay Eukaryotic DNA replication Simian virus 40 Biology Cell Transformation Viral beta-Galactosidase Pre-replication complex Virology Cell biology DNA replication factor CDT1 Epitopes Replication factor C Control of chromosome duplication biology.protein Origin recognition complex Cloning Molecular Viral Fusion Proteins Replication protein A Plasmids |
| Zdroj: | Philosophical Transactions of the Royal Society of London. B, Biological Sciences. 317:455-469 |
| ISSN: | 2054-0280 0080-4622 |
| Popis: | The small eukaryotic DNA tumour virus, SV40, has long provided a very useful model for the study of eukaryotic DNA replication and cellular transformation. The viral gene product, large-tumour (large-T) antigen, is essential for the initiation of viral DNA replication and the initiation and maintenance of SV40-virus-mediated cellular transformation. The large-T antigen is a complex multifunctional protein, and to delineate its activity more precisely in viral DNA replication and cellular transformation, small functional domains of the protein have been expressed in Escherichia coli and analysed by using a very extensive library of anti-T monoclonal antibodies. |
| Databáze: | OpenAIRE |
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