Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99
| Autor: | Malin Edén, Maria Hammond, Hanna Davies, Amanda Raine, Jonatan Halvardson, Jonas Mattisson, Martin Ingelsson, Marcus Danielsson, Jan P. Dumanski, Lars Forsberg, Jessica Nordlund, Caroline J. Gallant, Lena Kilander |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Medicin och hälsovetenskap Aging Cell- och molekylärbiologi Pseudoautosomal region Aneuploidy Diseases medicine.disease_cause Medical and Health Sciences Immunological techniques 0302 clinical medicine Leukocytes Aged 80 and over 0303 health sciences Mutation Multidisciplinary Mosaicism RNA sequencing 030220 oncology & carcinogenesis Medicine Single-Cell Analysis Medical Genetics Science CD99 Proteomic analysis Biology 12E7 Antigen Predictive markers Article 03 medical and health sciences Immune system Gene expression analysis Alzheimer Disease medicine Humans RNA Messenger Gene 030304 developmental biology Medicinsk genetik Aged Autosome Chromosomes Human Y Chromosome medicine.disease Molecular biology Cell and Molecular Biology Biomarkers |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Mosaic loss of chromosome Y (LOY) in immune cells is a male-specific mutation associated with increased risk for morbidity and mortality. TheCD99gene, positioned in the pseudoautosomal regions of chromosomes X and Y, encodes a cell surface protein essential for several key properties of leukocytes and immune system functions. Here we used CITE-seq for simultaneous quantification ofCD99derived mRNA and cell surface CD99 protein abundance in relation to LOY in single cells. The abundance of CD99 molecules was lower on the surfaces of LOY cells compared with cells without this aneuploidy in all six types of leukocytes studied, while the abundance of CD proteins encoded by genes located on autosomal chromosomes were independent from LOY. These results connect LOY in single cells with immune related cellular properties at the protein level, providing mechanistic insight regarding disease vulnerability in men affected with mosaic chromosome Y loss in blood leukocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink