Identification of a GLDH-overexpressing Arabidopsis mutant and its responses to high-light stress
Autor: | Chang-Lian Peng, M. Li, X.H. Zhang, Min-Ling Cai, Tai-Jie Zhang, Y.Z. Wang, X.T. Zheng |
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Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
Antioxidant Physiology medicine.medical_treatment Mutant Plant Science 01 natural sciences lcsh:Botany Arabidopsis medicine Chlorophyll fluorescence chemistry.chemical_classification Reactive oxygen species biology Chemistry Wild type 04 agricultural and veterinary sciences biology.organism_classification Ascorbic acid Molecular biology lcsh:QK1-989 Photoprotection 040103 agronomy & agriculture 0401 agriculture forestry and fisheries abiotic stress antioxidation ascorbate photoinhibition photoprotection 010606 plant biology & botany |
Zdroj: | Photosynthetica, Vol 57, Iss 1, Pp 332-341 (2019) |
ISSN: | 1573-9058 0300-3604 |
DOI: | 10.32615/ps.2019.038 |
Popis: | Ascorbic acid (AsA) is an important antioxidant protecting plant against environmental stresses. L-galactono-1,4-lactone dehydrogenase (GLDH) is a key enzyme in AsA synthesis pathway. To investigate the roles of AsA in mitigating high light (HL) damage, Arabidopsis GLDH mutants SALK_060087 and SALK_008236 with altered GLDH-expression were screened for homozygotes. No homozygotes were identified from SALK_060087, but most individuals of the SALK_008236 line (GLDH-236OE) were GLDH-overexpressing homozygous mutants accumulating more AsA than wild type (WT). An investigation of the physiological responses to HL demonstrated that the chlorophyll fluorescence parameters were significantly higher in GLDH-236OE than that in WT after 14-d HL. The degradation of photosynthetic pigment in WT was more severe than that in GLDH-236OE. GLDH-236OE accumulated more AsA, anthocyanins, flavonoids, and phenolics, while WT accumulated more reactive oxygen species (ROS) during HL. Our results suggest that GLDH-236OE have lesser sensitivity and higher tolerance to HL due to a higher capacity to eliminate ROS, absorb extra light, and dissipate thermal energy. |
Databáze: | OpenAIRE |
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