Enantioselective Study on the Biodegradation of Verapamil and Cytalopram by Chiral Capillary Electrophoresis
| Autor: | María José Medina-Hernández, Laura Escuder-Gilabert, Yolanda Martín-Biosca, Salvador Sagrado |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Salt (chemistry)
Filtration and Separation 010501 environmental sciences Citalopram 01 natural sciences biodegradation Analytical Chemistry lcsh:Chemistry Capillary electrophoresis enantioselectivity medicine 0105 earth and related environmental sciences chemistry.chemical_classification Chromatography 010401 analytical chemistry Enantioselective synthesis Biodegradation lcsh:QC1-999 0104 chemical sciences Activated sludge chemistry lcsh:QD1-999 Verapamil chiral capillary electrophoresis Enantiomer lcsh:Physics medicine.drug |
| Zdroj: | Separations, Vol 8, Iss 29, p 29 (2021) Separations Volume 8 Issue 3 |
| ISSN: | 2297-8739 |
| Popis: | Many of the currently available drugs are chiral compounds that are marketed as racemates or, to a lesser extent, in the form of one of the enantiomers since a pair of enantiomers may have different toxicological and ecotoxicological properties compared to each other. The evaluation of enantioselectivity in biodegradation processes is essential for environmental risk assessment. The objective of this research is to study the enantioselectivity in the biodegradation of two common chiral drugs, citalopram and verapamil, using highly sulphated-γ-cyclodextrin (HS-γ-CD) as chiral selector in Capillary Electrophoresis. Biodegradation experiments were performed in batch mode using a minimal salt medium inoculated with an activated sludge and supplemented with the corresponding enantiomeric mixture. The cultures were incubated at 20 °C for 28 days. Abiotic degradation of verapamil and citalopram enantiomers was also assessed. The concentration of the enantiomers of verapamil and citalopram were monitored using 0.7% and 0.1% m/v HS-γ-CD solutions as chiral selector, respectively. Separations were carried out using the complete filling technique. The results of biodegradability tests indicate that citalopram could be considered potentially persistent while verapamil is presumed to be a non-persistent compound. No evidence of enantioselectivity was observed in any of the biodegradation processes. |
| Databáze: | OpenAIRE |
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