Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets
| Autor: | Andrea Mückl, Friedrich C. Simmel, Matthaeus Schwarz-Schilling, Lukas Aufinger |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellular differentiation Green Fluorescent Proteins Microfluidics Biophysics Gene Expression Biology Biochemistry Diffusion 03 medical and health sciences chemistry.chemical_compound Synthetic biology Bacterial Proteins Gene expression Escherichia coli Inducer Micelles Microscopy Video Bacteria Cell-Free System Gene Expression Profiling Bacteria/genetics Bacterial Proteins/metabolism DNA/genetics Emulsions Escherichia coli/genetics Gene Expression Regulation Bacterial Green Fluorescent Proteins/chemistry Quorum Sensing Synthetic Biology/methods Transcription Factors/metabolism DNA biology.organism_classification Molecular biology ddc Gene expression profiling Quorum sensing 030104 developmental biology chemistry Synthetic Biology Transcription Factors |
| Zdroj: | Integrative biology : quantitative biosciences from nano to macro. 8(4) |
| ISSN: | 1757-9708 |
| Popis: | Position-dependent gene expression in gradients of morphogens is one of the key processes involved in cellular differentiation during development. Here, we study a simple artificial differentiation process, which is based on the diffusion of genetic inducers within one-dimensional arrangements of 50 \textgreek{m}m large water-in-oil droplets. The droplets are filled with either bacteria or cell-free gene expression systems, both equipped with genetic constructs that produce inducers or respond to them via expression of a fluorescent protein. We quantitatively study the coupled diffusion-gene expression process and demonstrate that gene expression can be made position-dependent both within bacteria-containing and cell-free droplets. By generating diffusing quorum sensing signals in situ, we also establish communication between artificial cell-free sender cells and bacterial receivers, and vice versa. |
| Databáze: | OpenAIRE |
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