Isolation of Legionella spp. from environmental water samples by low-pH treatment and use of a selective medium
| Autor: | G K Morris, C A Bopp, J G Wells, J W Sumner |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Microbiology (medical)
Cefalotin food.ingredient Legionella medicine.drug_class Cephalosporin Cycloheximide Animal Inoculation Legionella pneumophila Microbiology chemistry.chemical_compound food Vancomycin Cephalothin medicine Agar Chromatography biology Colistin Hydrogen-Ion Concentration bacterial infections and mycoses biology.organism_classification Culture Media respiratory tract diseases chemistry bacteria Water Microbiology Research Article medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 13:714-719 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.13.4.714-719.1981 |
| Popis: | A selective medium was developed and used successfully to isolate Legionella pneumophila and Legionella-like organisms from environmental specimens previously positive by animal inoculation methods. This medium consists of charcoal-yeast extract agar to which have been added cephalothin (4 micrograms/ml), colistin (16 micrograms/ml), vancomycin (0.5 microgram/ml), and cycloheximide (80 micrograms/ml). Pretreating of the environmental water samples with an acid buffer (pH 2.2), followed by plating on the selective medium, improved the rate of recovery of both Legionella and Legionella-like organisms relative to that with direct plating on selective media. |
| Databáze: | OpenAIRE |
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