An implanted recombination hot spot stimulates recombination and enhances sister chromatid cohesion of heterologous YACs during yeast meiosis
| Autor: | Dorothy D. Sears, G Simchen, Philip Hieter |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
FLP-FRT recombination
Molecular Sequence Data DNA Recombinant Saccharomyces cerevisiae Investigations Biology Genetic recombination Chromosomal crossover Meiosis Genetics Humans Sister chromatids DNA Fungal Chromosomes Artificial Yeast Recombination Genetic Base Sequence fungi Molecular biology Establishment of sister chromatid cohesion Nondisjunction biological phenomena cell phenomena and immunity Chromosomes Fungal Homologous recombination Sister Chromatid Exchange |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/138.4.1055 |
| Popis: | Heterologous yeast artificial chromosomes (YACs) do not recombine with each other and missegregate in 25% of meiosis I events. Recombination hot spots in the yeast Saccharomyces cerevisiae have previously been shown to be associated with sites of meiosis-induced double-strand breaks (DSBs). A 6-kb fragment containing a recombination hot spot/DSB site was implanted onto two heterologous human DNA YACs and was shown to cause the YACs to undergo meiotic recombination in 5-8% of tetrads. Reciprocal exchanges initiated and resolved within the 6-kb insert. Presence of the insert had no detectable effect on meiosis I nondisjunction. Surprisingly, the recombination hot spots acted in cis to significantly reduce precocious sister-chromatid segregation. This novel observation suggests that DSBs are instrumental in maintaining cohesion between sister chromatids in meiosis I. We propose that this previously unknown function of DSBs is mediated by the stimulation of sister-chromatid exchange and/or its intermediates. |
| Databáze: | OpenAIRE |
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