Biologically Inspired Scaffolds for Heart Valve Tissue Engineering via Melt Electrowriting
| Autor: | Petra Mela, Frederic Wolf, Navid T. Saidy, Elena M. De-Juan-Pardo, Hans Keijdener, Dietmar W. Hutmacher, Onur Bas |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Biocompatibility Polymers Myocytes Smooth Muscle Heart Valve Diseases Biomimetic design 02 engineering and technology 010402 general chemistry 01 natural sciences Viscoelasticity Umbilical Cord Biomaterials Extracellular matrix Heart valve tissue engineering Biomimetics Materials Testing medicine Humans General Materials Science Heart valve Cells Cultured Tissue Engineering Tissue Scaffolds Guided Tissue Regeneration Infant Newborn Strain stiffening General Chemistry 021001 nanoscience & nanotechnology Electroplating Heart Valves 0104 chemical sciences Biomechanical Phenomena Blood Vessel Prosthesis medicine.anatomical_structure Printing Three-Dimensional 0210 nano-technology Biotechnology Biomedical engineering Biofabrication |
| Zdroj: | Small (Weinheim an der Bergstrasse, Germany). 15(24) |
| ISSN: | 1613-6829 |
| Popis: | Heart valves are characterized to be highly flexible yet tough, and exhibit complex deformation characteristics such as nonlinearity, anisotropy, and viscoelasticity, which are, at best, only partially recapitulated in scaffolds for heart valve tissue engineering (HVTE). These biomechanical features are dictated by the structural properties and microarchitecture of the major tissue constituents, in particular collagen fibers. In this study, the unique capabilities of melt electrowriting (MEW) are exploited to create functional scaffolds with highly controlled fibrous microarchitectures mimicking the wavy nature of the collagen fibers and their load-dependent recruitment. Scaffolds with precisely-defined serpentine architectures reproduce the J-shaped strain stiffening, anisotropic and viscoelastic behavior of native heart valve leaflets, as demonstrated by quasistatic and dynamic mechanical characterization. They also support the growth of human vascular smooth muscle cells seeded both directly or encapsulated in fibrin, and promote the deposition of valvular extracellular matrix components. Finally, proof-of-principle MEW trileaflet valves display excellent acute hydrodynamic performance under aortic physiological conditions in a custom-made flow loop. The convergence of MEW and a biomimetic design approach enables a new paradigm for the manufacturing of scaffolds with highly controlled microarchitectures, biocompatibility, and stringent nonlinear and anisotropic mechanical properties required for HVTE. |
| Databáze: | OpenAIRE |
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