Microspectrophotometric Dna Analysis of Malignant Salivary Gland Tumours
| Autor: | A Zetterberg, C M Eneroth |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Adenocarcinoma Biology chemistry.chemical_compound Biopsy medicine Carcinoma Humans Parotid Gland Lymphocytes Feulgen stain Cell Nucleus Staining and Labeling Salivary gland medicine.diagnostic_test Biopsy Needle DNA Neoplasm General Medicine medicine.disease Carcinoma Adenoid Cystic Parotid Neoplasms Nuclear DNA Staining medicine.anatomical_structure Otorhinolaryngology chemistry Spectrophotometry DNA |
| Zdroj: | Acta Oto-Laryngologica. 77:289-294 |
| ISSN: | 1651-2251 0001-6489 |
| DOI: | 10.3109/00016487409124627 |
| Popis: | Microspectrophotometric determinations of nuclear DNA quantity of Feulgen-stained cells were made in six selected malignant salivary gland tumours and compared to normal salivary gland tissue and human lymphocytes from peripheral blood. Two questions of methodical importance were investigated and it was found that no errors in the quantitative DNA determination were introduced as a result of the imprint technique and that the optimal duration of acid hydrolysis in the Feulgen staining procedure was 60 min. Microspectrophotometric DNA analysis of the six malignant salivary gland tumours showed nuclear DNA quantities which exceeded that of the normal control cells irrespective of the type of tumor and degree of differentiation. Furthermore, a much higher degree of intercellular variability with respect to the nuclear DNA content was found in the malignant tumour cell populations. The fraction of cell nuclei with DNA values exceeding that of normal cells varied according to the type of tumour, and the incide... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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