Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and pro-inflammation assays for nanoparticles
Autor: | Luciene Bottentuit Balottin, Albert Duschl, Federico Benetti, Andrea Haase, Monica Simion, Matthew S. P. Boyles, Paulo Emílio Corrêa Leite, Olesja Bondarenko, Olivier Toussaint, Ulrike Tischler, Braulio H.F. Lima, Christelle Saout, Anne Kahru, Superb K. Misra, Inge Nelissen, An Jacobs, José Mauro Granjeiro, Ana R. Ribeiro, Jean-Pascal Piret, Elise Dumortier, Isabella Radauer-Preiml, Sergio Anguissola, Angela Ivask, Annegret Potthoff, Martin Himly, Caroline Smal |
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Přispěvatelé: | Publica, Piret, Jean-Pascal, Bondarenko, Olesja M, Boyles, Matthew SP, Himly, Martin, Ivask, Angela, Toussaint, Olivier |
Rok vydání: | 2016 |
Předmět: |
Silver
Pro inflammation Serial dilution Cell Survival Surface Properties Health Toxicology and Mutagenesis inter-laboratory studies Cell interference in vitro assays Metal Nanoparticles Nanoparticle Nanotechnology 02 engineering and technology 010501 environmental sciences Toxicology 01 natural sciences inter-laboratory study Cell Line Tumor Inter-laboratory studies Toxicity Tests medicine Humans Viability assay Particle Size standard operating procedures 0105 earth and related environmental sciences Chemistry nanoparticle In vitro toxicology Reproducibility of Results General Medicine 021001 nanoscience & nanotechnology Europe Standard operating procedures medicine.anatomical_structure Biochemistry Cell culture Toxicity In vitro assays Cytokines Nanoparticles nanoparticles Biological Assay In vitro assay Laboratories Interference 0210 nano-technology Copper |
Zdroj: | Archives of Toxicology. 91:2315-2330 |
ISSN: | 1432-0738 0340-5761 |
Popis: | The rapid development of nanotechnologies and increased production and use of nanomaterials raise concerns about their potential toxic effects for human health and environment. To evaluate the biological effects of nanomaterials, a set of reliable and reproducible methods and development of standard operating procedures (SOPs) is required. In the framework of the European FP7 NanoValid project, three different cell viability assays (MTS, ATP content, and caspase-3/7 activity) with different readouts (absorbance, luminescence and fluorescence) and two immune assays (ELISA of pro-inflammatory cytokines IL1-β and TNF-α) were evaluated by inter-laboratory comparison. The aim was to determine the suitability and reliability of these assays for nanosafety assessment. Studies on silver and copper oxide nanoparticles (NPs) were performed, and SOPs for particle handling, cell culture, and in vitro assays were established or adapted. These SOPs give precise descriptions of assay procedures, cell culture/seeding conditions, NPs/positive control preparation and dilutions, experimental well plate preparation, and evaluation of NPs interference. The following conclusions can be highlighted from the pan-European inter-laboratory studies: Testing of NPs interference with the toxicity assays should always be conducted. Interference tests should be designed as close as possible to the cell exposure conditions. ATP and MTS assays gave consistent toxicity results with low inter-laboratory variability using Ag and CuO NPs and different cell lines and therefore, could be recommended for further validation and standardization. High inter-laboratory variability was observed for Caspase 3/7 assay and ELISA for IL1-β and TNF-α measurements. by Superb K. Misra et al. |
Databáze: | OpenAIRE |
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