Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and pro-inflammation assays for nanoparticles

Autor: Luciene Bottentuit Balottin, Albert Duschl, Federico Benetti, Andrea Haase, Monica Simion, Matthew S. P. Boyles, Paulo Emílio Corrêa Leite, Olesja Bondarenko, Olivier Toussaint, Ulrike Tischler, Braulio H.F. Lima, Christelle Saout, Anne Kahru, Superb K. Misra, Inge Nelissen, An Jacobs, José Mauro Granjeiro, Ana R. Ribeiro, Jean-Pascal Piret, Elise Dumortier, Isabella Radauer-Preiml, Sergio Anguissola, Angela Ivask, Annegret Potthoff, Martin Himly, Caroline Smal
Přispěvatelé: Publica, Piret, Jean-Pascal, Bondarenko, Olesja M, Boyles, Matthew SP, Himly, Martin, Ivask, Angela, Toussaint, Olivier
Rok vydání: 2016
Předmět:
Silver
Pro inflammation
Serial dilution
Cell Survival
Surface Properties
Health
Toxicology and Mutagenesis
inter-laboratory studies
Cell
interference
in vitro assays
Metal Nanoparticles
Nanoparticle
Nanotechnology
02 engineering and technology
010501 environmental sciences
Toxicology
01 natural sciences
inter-laboratory study
Cell Line
Tumor
Inter-laboratory studies
Toxicity Tests
medicine
Humans
Viability assay
Particle Size
standard operating procedures
0105 earth and related environmental sciences
Chemistry
nanoparticle
In vitro toxicology
Reproducibility of Results
General Medicine
021001 nanoscience & nanotechnology
Europe
Standard operating procedures
medicine.anatomical_structure
Biochemistry
Cell culture
Toxicity
In vitro assays
Cytokines
Nanoparticles
nanoparticles
Biological Assay
In vitro assay
Laboratories
Interference
0210 nano-technology
Copper
Zdroj: Archives of Toxicology. 91:2315-2330
ISSN: 1432-0738
0340-5761
Popis: The rapid development of nanotechnologies and increased production and use of nanomaterials raise concerns about their potential toxic effects for human health and environment. To evaluate the biological effects of nanomaterials, a set of reliable and reproducible methods and development of standard operating procedures (SOPs) is required. In the framework of the European FP7 NanoValid project, three different cell viability assays (MTS, ATP content, and caspase-3/7 activity) with different readouts (absorbance, luminescence and fluorescence) and two immune assays (ELISA of pro-inflammatory cytokines IL1-β and TNF-α) were evaluated by inter-laboratory comparison. The aim was to determine the suitability and reliability of these assays for nanosafety assessment. Studies on silver and copper oxide nanoparticles (NPs) were performed, and SOPs for particle handling, cell culture, and in vitro assays were established or adapted. These SOPs give precise descriptions of assay procedures, cell culture/seeding conditions, NPs/positive control preparation and dilutions, experimental well plate preparation, and evaluation of NPs interference. The following conclusions can be highlighted from the pan-European inter-laboratory studies: Testing of NPs interference with the toxicity assays should always be conducted. Interference tests should be designed as close as possible to the cell exposure conditions. ATP and MTS assays gave consistent toxicity results with low inter-laboratory variability using Ag and CuO NPs and different cell lines and therefore, could be recommended for further validation and standardization. High inter-laboratory variability was observed for Caspase 3/7 assay and ELISA for IL1-β and TNF-α measurements.
by Superb K. Misra et al.
Databáze: OpenAIRE
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