Detection and quantitation of specific mRNAs by ribonuclease protection assay using denaturing horizontal polyacrylamide gel electrophoresis: A radioactive and nonradioactive approach
| Autor: | Friedolf Peters, Ralf Einspanier, Annette Plath |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Gel electrophoresis
Chromatography Gel electrophoresis of nucleic acids Clinical Biochemistry Polyacrylamide Transforming Growth Factor alpha Gel electrophoresis of proteins complex mixtures Biochemistry Analytical Chemistry enzymes and coenzymes (carbohydrates) chemistry.chemical_compound Electrophoresis Ribonucleases chemistry Molecular-weight size marker Animals Digoxigenin Cattle Electrophoresis Polyacrylamide Gel Fibroblast Growth Factor 2 RNA Messenger Insulin-Like Growth Factor I Polyacrylamide gel electrophoresis |
| Zdroj: | Electrophoresis. 17:471-472 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.1150170306 |
| Popis: | A radioactive (32P) and nonradioactive (digoxigenin) ribonuclease protection assay (RPA) has been developed to detect mRNAs of housekeeping proteins and growth factors. A modification of polyacrylamide gel electrophoresis (PAGE) to simplify RPA is described. Both Cleangels (Pharmacia) and laboratory-cast polyacrylamide gels, in a denaturing, horizontal electrophoresis system, were used. The amount of toxic chemicals and waste was reduced, in comparison with sequencing gels normally used for RPA. The protected RNA fragments were shown to be well-separated, with sufficient sensitivity in this modified, quick gel system. |
| Databáze: | OpenAIRE |
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