Occurrence and a possible mechanism of penetration of natural killer cells into K562 target cells during the cytotoxic interaction

Autor: Radosevic, Katarina, Segers-Nolten, Gezina M.J., van Leeuwen, Anne Marie T., Figdor, Carl, de Grooth, B.G., Greve, Jan, Radosevic, K.
Přispěvatelé: Faculty of Science and Technology, Nanobiophysics
Rok vydání: 1995
Předmět:
Cytotoxicity
Immunologic
METIS-129718
Cytochalasin D
Biophysics
chemistry.chemical_element
Apoptosis
macromolecular substances
Calcium
Biology
Natural Killer cell
Pathology and Forensic Medicine
Natural killer cell
endonuclease activity
Cell Fusion
chemistry.chemical_compound
Endocrinology
Biopolymers
The role of P150
95 leukocyte adhesion receptors in the inflammatory/immune respons
Fluorescence microscope
medicine
Cell Adhesion
Tumor Cells
Cultured
Cytotoxic T cell
Humans
De rol van P150
95 leukoccyt adhesie receptor in de ontstekings/immuun respons
emperipolesis
Actin
IR-60720
Microscopy
Confocal
actin polymerization
Sulfates
Cell Biology
Hematology
Endonucleases
Actins
Zinc Sulfate
Cell biology
Killer Cells
Natural
medicine.anatomical_structure
chemistry
Polymerization
Microscopy
Fluorescence
Zinc Compounds
Leukemia
Erythroblastic
Acute
K562 cells
Zdroj: Cytometry, 20, 273-280
Cytometry, 20, 4, pp. 273-280
Cytometry, 20, pp. 273-280
Cytometry, 20, 273-280. Wiley-liss
Cytometry, 20(20), 273-280. Wiley
ISSN: 0196-4763
Popis: The cytotoxic interaction between cloned human Natural Killer (NK) cells and K562 target cells was studied using confocal laser scanning microscopy (CLSM) and conventional fluorescence microscopy. We observed, using fixed as well as living cells, the occurrence of (pseudo)emperipolesis during the interaction. About 30% of conjugated NK cells penetrated, partly or completely, into the target cells (in-conjugation). Virtually all in-conjugated target cells exhibited polymerized actin. Killer cells of in-conjugates were frequently seen approaching the target cell nucleus or aligning along it. If the cytotoxic process was inhibited by the absence of calcium neither actin polymerization nor in-conjugation were observed. A kinetic study showed that in-conjugation starts somewhat later than actin polymerization but still within a few minutes after addition of calcium to conjugates previously formed in the absence of calcium. The presence of cytochalasin D (an inhibitor of actin polymerization) completely inhibited in-conjugation and partly reduced the cytotoxic activity. Zinc ions (endonuclease inhibition) inhibited in-conjugation and decreased the total number of target cells with polymerized actin in a concentration dependent manner. Cytotoxic activity was also reduced but not as efficiently as in-conjugation. Our study demonstrates that in-conjugation represents a significant fraction of the cytotoxic interaction. The results indicate that it may be a consequence of an actin polymerization and endonuclease activity dependent part of a cytotoxic mechanism.
Databáze: OpenAIRE
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