Interaction between the plasmid R6K andEscherichia coliwith defective DNA polymerase I
| Autor: | D. J. Tweats, J. T. Smith |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
DNA Replication
DNA clamp biology Chemistry DNA polymerase R Factors DNA polymerase II DNA-Directed DNA Polymerase General Medicine DNA Polymerase I Molecular biology Real-time polymerase chain reaction Escherichia coli Genetics biology.protein Primase Primer (molecular biology) DNA polymerase I Polymerase |
| Zdroj: | Genetical Research. 32:25-35 |
| ISSN: | 1469-5073 0016-6723 |
| Popis: | SUMMARYInitial experiments demonstrated that the plasmid R6K cannot be transferred to or maintained readily in theE. coliDNA polymerase I deficient strain JG138polA1. Results withE. coliMM386polA12(R6K), which has a temperature sensitive polymerase I enzyme, showed cell division becomes abnormal when the polymerase I enzyme of the host bacteria is inactivated at the restrictive temperature. Under conditions of polymerase I deficiency, R6K replication, as measured by monitoring R-factor-mediated β-lactamase activity, also becomes abnormal with the loss of multiple R6K copies per cell and the apparent maintenance of a single R-factor copy per cell. |
| Databáze: | OpenAIRE |
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