Viral Strategies for Targeting the Central and Peripheral Nervous Systems
| Autor: | Benjamin E. Deverman, Claire N. Bedbrook, Viviana Gradinaru |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Central Nervous System
0301 basic medicine Nervous system Cell type viruses Transgene Biology medicine.disease_cause Virus 03 medical and health sciences 0302 clinical medicine Peripheral Nervous System medicine Animals Humans Gene Adeno-associated virus General Neuroscience Dependovirus Cell biology 030104 developmental biology medicine.anatomical_structure Capsid Tissue tropism Genetic Engineering Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Annual Review of Neuroscience. 41:323-348 |
| ISSN: | 1545-4126 0147-006X |
| DOI: | 10.1146/annurev-neuro-080317-062048 |
| Popis: | Recombinant viruses allow for targeted transgene expression in specific cell populations throughout the nervous system. The adeno-associated virus (AAV) is among the most commonly used viruses for neuroscience research. Recombinant AAVs (rAAVs) are highly versatile and can package most cargo composed of desired genes within the capsid's ∼5-kb carrying capacity. Numerous regulatory elements and intersectional strategies have been validated in rAAVs to enable cell type–specific expression. rAAVs can be delivered to specific neuronal populations or globally throughout the animal. The AAV capsids have natural cell type or tissue tropism and trafficking that can be modified for increased specificity. Here, we describe recently engineered AAV capsids and associated cargo that have extended the utility of AAVs in targeting molecularly defined neurons throughout the nervous system, which will further facilitate neuronal circuit interrogation and discovery. |
| Databáze: | OpenAIRE |
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