An optimized controlled rate slow cooling protocol for bovine ovarian tissue cryopreservation by means of X-ray computed tomography
| Autor: | Ángel Parrado-Gallego, Christiani Andrade Amorim, Miguel Gallardo, Ramón Risco, Ariadna Corral, Marcin Balcerzyk |
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| Přispěvatelé: | Junta de Andalucía, Siemens Healthcare, Ministerio de Economía y Competitividad (España), Balcerzyk, Marcin [0000-0001-6030-7416], Amorim, Christiani A. [0000-0003-1794-0368], Risco, Ramón [0000-0001-5995-6590], UCL - SSS/IREC/GYNE - Pôle de Gynécologie, Balcerzyk, Marcin, Amorim, Christiani A., Risco, Ramón |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Time Factors Cryoprotectant Population Cryopreservation Cryoprotectant visualization 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Food Animals Animals Ovarian tissue cryopreservation Fertility preservation education Small Animals education.field_of_study 030219 obstetrics & reproductive medicine Dimethyl sulfoxide Equine Tissue permeation Methanol Ovary Permeation Cold Temperature Transplantation 030104 developmental biology chemistry CT imaging Cattle Female Animal Science and Zoology Tissue Preservation Tomography X-Ray Computed Biomedical engineering |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Theriogenology, Vol. 119, p. 183-188 (2018) |
| Popis: | Cryopreservation and subsequent transplantation of ovarian tissue is the only option to preserve fertility in certain patients facing gonadotoxic treatment. So far, cryopreservation of ovarian tissue has been carried out mostly by a controlled rate slow cooling process, typically known as slow freezing. Even though there are still some concerns about the iatrogenic damage on the follicle population, this technique has been used in the more than 100 live births reported to date. It is well known that the control of the cryoprotectant loading in the tissue is crucial to in a cryopreservation procedure. We have used the technology of X-ray computed tomography to assess the concentration and distribution of dimethyl sulfoxide (one of the cryoprotectants most used in fertility preservation) inside pieces of bovine ovarian tissue after its cryopreservation. The low voltage used in our device (75 kV) and the high electronic density of this cryoprotectant makes the X-ray attenuation proportional to its concentration. By assessing and comparing the permeation and homogeneity of the cryoprotectant inside ovarian tissue fragments subjected to a controlled rate slow cooling process, we have characterized the effect of variations in the main parameters involved in the process, with the goal of achieving an optimized protocol with higher permeation of the cryoprotectant in the tissue. The most promissory results were obtained by increasing the initial concentration of dimethyl sulfoxide in the vehicle solution from 10 to 20. This work has been partially supported by the Junta de Andalucía, Proyectos de Investigación de Excelencia (P08-CTS-03965), Siemens Healthcare S.L.U. (2729/0708) and Retos-Colaboración RTC-2016-4733-1 (MINECO, Spain). |
| Databáze: | OpenAIRE |
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