E-cadherin expression in the subepithelial nevus cells of the giant congenital nevocellular nevi (GCNN) correlates with their migration ability in vitro
| Autor: | Tatsuo Nakajima, Hideo Nakajima, Miho Mukai, Yuko Katsuki, Akira Yamaguchi, Shuji Mikami, Hachiro Iseki, Ken-ichi Katsube, Yoshiro Takano, N. Matsuda, Kazuo Kishi |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Skin Neoplasms Population Dermatology Biology Biochemistry Dermis Cell Movement Intradermal Nevus medicine Nevus Humans skin and connective tissue diseases education Microscopy Immunoelectron Molecular Biology beta Catenin education.field_of_study Epidermis (botany) Cadherin Infant Cell migration Epithelial Cells medicine.disease Cadherins medicine.anatomical_structure Epidermal Cells Child Preschool Nevus Intradermal Epidermis Nevus cell |
| Zdroj: | Journal of dermatological science. 52(1) |
| ISSN: | 0923-1811 |
| Popis: | Summary Background Giant congenital nevocellular nevi (GCNN) are histologically characterized by the broad distribution of nevus cells in the epidermis and dermis. Objective To characterize E-cadherin in GCNN and define its role in nevic cell migrations. Methods Twenty-four cases were immunohistochemically examined and in five cases cells were isolated for primary culture for migration assays. Results The nevus cells in the superficial region showed the immunoreactivity of E-cadherin in a membranous pattern, but those in the deep part of dermis had little immunoreactivity. Ultra-structural analysis of the superficial nevus cells revealed that E-cadherin immunodeposits in the fibrillar processes around the cell body in a spotted pattern. This distribution pattern is quite different from that in the adherens junction of skin squamous epithelial cells. Boyden chamber experiments were performed using primary cultures of intradermal nevus cells. EDTA pretreatment reduced cell migration to the E-cadherin positive side when the E-cadherin positive population was relatively large in the primary cultures. Conclusions These results indicate that E-cadherin in the nevus cells may affect nevus cell motility rather than intercellular attachment. |
| Databáze: | OpenAIRE |
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