Protocol to Fabricate Engineered Illumination Devices for Optogenetic Control of Cellular Signaling Dynamics
| Autor: | Hunter J. Johnson, Ravi S. Kane, Thomas McClave, David V. Schaffer, Nicole A. Repina |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell signaling
Light General Immunology and Microbiology Computer science business.industry General Neuroscience Cell Culture Techniques Throughput Optogenetics General Biochemistry Genetics and Molecular Biology Protocol Light activation business lcsh:Science (General) Protocol (object-oriented programming) Lighting Computer hardware Signal Transduction lcsh:Q1-390 |
| Zdroj: | STAR Protocols, Vol 1, Iss 3, Pp 100141-(2020) STAR Protocols |
| ISSN: | 2666-1667 |
| Popis: | Summary Optogenetic modulation of protein interactions enables spatiotemporal control of cellular signaling dynamics in a variety of biological systems. However, light patterning by standard microscopes is limited by their complexity, sample throughput, and cost. To address the need for low-cost, user-friendly, and high-throughput photopatterning, we have engineered devices for light activation at variable amplitudes (LAVA). This protocol describes the assembly of LAVA devices, which enable spatial and temporal control of optogenetic stimulation and cellular signaling dynamics in multiwell cell culture plates. For complete details on the use and execution of this protocol, please refer to Repina et al. (2020). Graphical Abstract Highlights • Fabrication of illumination devices for light activation at variable amplitudes (LAVA) • Spatiotemporal control of light illumination in multiwell cell culture plates • Overview of optogenetic photostimulation and analysis in LAVA devices Optogenetic modulation of protein interactions enables spatiotemporal control of cellular signaling dynamics in a variety of biological systems. However, light patterning by standard microscopes is limited by their complexity, sample throughput, and cost. To address the need for low-cost, user-friendly, and high-throughput photopatterning, we have engineered devices for light activation at variable amplitudes (LAVA). This protocol describes the assembly of LAVA devices, which enable spatial and temporal control of optogenetic stimulation and cellular signaling dynamics in multiwell cell culture plates. |
| Databáze: | OpenAIRE |
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