Investigation of Factors Affecting Controlled Release from Photosensitive DMPC and DSPC Liposomes
| Autor: | Ashok Kumar, Kathryn Torrey, Larry D. Stephenson, Aysegul Aygun |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Indoles
Lysis Light Lactose Bioengineering Isoindoles Applied Microbiology and Biotechnology Biochemistry Nitrophenols Hydrolysis Broad spectrum Organometallic Compounds Photodegradation Molecular Biology Liposome Photosensitizing Agents Chromatography Chemistry Photodissociation Temperature technology industry and agriculture Galactose General Medicine Nitrophenylgalactosides beta-Galactosidase Controlled release Cholesterol Glucose Zinc Compounds Delayed-Action Preparations Liposomes Phosphatidylcholines Biophysics Colorimetry lipids (amino acids peptides and proteins) Dimyristoylphosphatidylcholine Biotechnology Visible spectrum |
| Zdroj: | Applied Biochemistry and Biotechnology. 167:743-757 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-012-9724-6 |
| Popis: | An investigation of liposomes comprised of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) or 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) lipids with cholesterol and zinc phthalocyanine (ZnPC) revealed that several fundamental liposome properties are influenced by composition and by lipid-specific features. DMPC and DSPC liposomes were synthesized, and their compositional changes, encapsulation capacities, morphologies, and release properties were evaluated. In this research, liposome degradation, lysis, and content release were initiated by photolysis, i.e., rupture induced by exposure to light. A controlled release mechanism was created through the introduction of photosensitizers (i.e., ZnPC) embedded within the cholesterol-stabilized liposome membrane. The light wavelength and light exposure time accelerated photodegradation properties of DMPC liposomes compared to DSPC liposomes, which exhibited a slower release rate. Morphological changes in the liposomes were strongly influenced by light wavelength and light exposure time. For both the DMPC and DSPC liposomes, visible light with wavelengths in the red end of the spectrum and broad spectrum ambient lighting (400-700 nm) were more effective for lysis than UV-A light (365 nm). Heating liposomes to 100 °C decreased the stability of liposomes compared to liposomes kept at room temperatures. In addition, the optimal lipid-to-cholesterol-to-photoactivator ratio that produced the most stable liposomes was determined. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink