Three-dimensional reconstruction of bovine intradural spinal root myelin by electron microscope tomography
| Autor: | L.-G. Öfverstedt, U. Skoglund, J. Sedzik |
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| Rok vydání: | 1992 |
| Předmět: |
Cytoplasm
Materials science Stain Models Biological law.invention Cellular and Molecular Neuroscience chemistry.chemical_compound Myelin Nuclear magnetic resonance law medicine Image Processing Computer-Assisted Animals Tomography Myelin Sheath Staining and Labeling Resolution (electron density) Anatomy Staining Microscopy Electron medicine.anatomical_structure Osmium tetroxide chemistry Spinal Cord Cattle Electron microscope Electron Microscope Tomography |
| Zdroj: | Journal of neuroscience research. 31(2) |
| ISSN: | 0360-4012 |
| Popis: | Electron microscope tomography was used to reconstruct three-dimensionally the configuration of heavy metal staining in bovine intradural spinal root myelin. Samples were fixed with glutaraldehyde, exposed to osmium tetroxide, embedded, thin sectioned, and finally stained with uranyl-acetate and lead citrate. Reconstructions up to 4.2 nm resolution showed a non-uniform distribution of stain in the planes of individual cytoplasmfc appositions (major dense lines). In each reconstructed major dense line the stain was distributed in striated, well-defined structures. Those structures appear to be nearly parallel between neighboring major dense lines. The distribution of stain in the Schmidt-Lanterman cleft did not resemble the distribution of stain in the major dense line; however, weak striations were present. Evidence that the striated structures are not an artifact due to image calculation is discussed. |
| Databáze: | OpenAIRE |
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