Recombinant human fibroblast growth factor-2 promotes nerve regeneration and functional recovery after mental nerve crush injury
| Autor: | NaRi Seo, Jong-Ho Lee, Bongju Kim, Sung Ho Lee, Soung Min Kim, Wei-Peng Jin, Kang-Mi Pang |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty functional recovery sensory neuron Nerve guidance conduit lcsh:RC346-429 03 medical and health sciences Trigeminal ganglion 0302 clinical medicine Developmental Neuroscience Internal medicine fibroblast growth factor medicine Axon nerve regeneration mental nerve crush injury neural regeneration lcsh:Neurology. Diseases of the nervous system business.industry Regeneration (biology) medicine.disease Mental nerve Sensory neuron Neuronal tracing 030104 developmental biology medicine.anatomical_structure Endocrinology Crush injury business 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research, Vol 12, Iss 4, Pp 629-636 (2017) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| Popis: | Several studies have shown that fibroblast growth factor-2 (FGF2) can directly affect axon regeneration after peripheral nerve damage. In this study, we performed sensory tests and histological analyses to study the effect of recombinant human FGF-2 (rhFGF2) treatment on damaged mental nerves. The mental nerves of 6-week-old male Sprague-Dawley rats were crush-injured for 1 minute and then treated with 10 or 50 μg/mL rhFGF2 or PBS in crush injury area with a mini Osmotic pump. Sensory test using von Frey filaments at 1 week revealed the presence of sensory degeneration based on decreased gap score and increased difference score. However, at 2 weeks, the gap score and difference score were significantly rebounded in the mental nerve crush group treated with 10 μg/mL rhFGF2. Interestingly, treatment with 10 μg/mL rhFGF had a more obviously positive effect on the gap score than treatment with 50 μg/mL rhFGF2. In addition, retrograde neuronal tracing with Dil revealed a significant increase in nerve regeneration in the trigeminal ganglion at 2 and 4 weeks in the rhFGF2 groups (10 μg/mL and 50 μg/mL) than in the PBS group. The 10 μg/mL rhFGF2 group also showed an obviously robust regeneration in axon density in the mental nerve at 4 weeks. Our results demonstrate that 10 μg/mL rhFGF induces mental nerve regeneration and sensory recovery after mental nerve crush injury. |
| Databáze: | OpenAIRE |
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