Two-dimensional gel electrophoretic detection of protein carbonyls derivatized with biotin-hydrazide
| Autor: | Liang-Jun Yan, Jinzi Wu, Siqun Jing, Xiaoting Luo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Streptavidin Male Clinical Biochemistry Biotin Biotin hydrazide Mitochondrion Protein oxidation Biochemistry Article Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Testis Animals Electrophoresis Gel Two-Dimensional Reactive nitrogen species Chromatography Isoelectric focusing Proteins Cell Biology General Medicine Mitochondria Rats 030104 developmental biology chemistry Carbonylation Oxidation-Reduction |
| Popis: | Protein carbonyls are protein oxidation products that are often used to measure the magnitude of protein oxidative damage induced by reactive oxygen or reactive nitrogen species. Protein carbonyls have been found to be elevated during aging and in age-related diseases such as stroke, diabetes, and neurodegenerative diseases. In the present article, we provide detailed protocols for detection of mitochondrial protein carbonyls labeled with biotin-hydrazide followed by 2-dimensional isoelectric focusing (IEF)/SDS-PAGE and Western blotting probed with horse-radish peroxidase-conjugated streptavidin. The presented procedures can also be modified for detection of carbonylation of non-mitochondrial proteins. |
| Databáze: | OpenAIRE |
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