Antibacterial Activity and Characterization of Bacteria Isolated from Diverse Types of Greek Honey against Nosocomial and Foodborne Pathogens
| Autor: | Spyros Pournaras, Marios Nikolaidis, Christina Tsadila, T.G. Dimitriou, Ioannis Kafantaris, Dimitris Mossialos, Grigoris D. Amoutzias |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Technology Salmonella QH301-705.5 QC1-999 polyketide synthase 030106 microbiology honey Biology medicine.disease_cause Microbiology 03 medical and health sciences Nonribosomal peptide medicine microbiota Food microbiology General Materials Science Biology (General) QD1-999 Instrumentation Fluid Flow and Transfer Processes chemistry.chemical_classification antimicrobial activity 16S-rRNA gene Pseudomonas aeruginosa secondary metabolites Physics Process Chemistry and Technology General Engineering Engineering (General). Civil engineering (General) Antimicrobial biology.organism_classification Computer Science Applications Acinetobacter baumannii Citrobacter freundii Chemistry 030104 developmental biology chemistry TA1-2040 nonribosomal peptide synthetase Bacteria |
| Zdroj: | Applied Sciences Volume 11 Issue 13 Applied Sciences, Vol 11, Iss 5801, p 5801 (2021) |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app11135801 |
| Popis: | It has been suggested that microorganisms present in honey are a potential source of antimicrobial compounds. This study aimed to isolate and characterize bacteria from 46 Greek honey samples of diverse botanical and geographical origin and to determine whether these bacteria demonstrate antibacterial activity against five important nosocomial and foodborne pathogens. In total, 2014 bacterial isolates were obtained and screened for antibacterial activity. Overall, 16% of the isolates inhibited the growth of Staphylococcus aureus, 11.2% inhibited the growth of Pseudomonas aeruginosa and Acinetobacter baumannii, 10.2% inhibited the growth of Salmonella Typhimurium and 12.4% of the isolates affected the growth of Citrobacter freundii. In total, 316 isolates that inhibited the growth of more than two of the tested pathogens were grouped by restriction fragment length polymorphisms (RFLP) analysis of the 16S rRNA gene amplicon. Fifty of them were identified by 16S rRNA gene sequencing. The majority, 62% of the isolates, belonged to the genus Bacillus. Only 10% of the isolates were identified as Gram-negative bacteria. Furthermore, in several bacterial isolates, genes encoding polyketide synthases and nonribosomal peptide synthetases that catalyze the biosynthesis of secondary metabolites which might contribute to the exerted antimicrobial activity, were detected. This study demonstrates that honey microbiota exerts antimicrobial activity and is a putative source of secondary metabolites against important nosocomial and food pathogens that warrants further investigation. |
| Databáze: | OpenAIRE |
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