Correlation of exons with functional domains and folding regions in a cellulase from Agaricus bisporus
| Autor: | Christopher F. Thurston, Michael P. Challen, C.-M. Chow, Ernesto Yagüe |
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| Rok vydání: | 1996 |
| Předmět: |
Protein Folding
Agaricus Genes Fungal Molecular Sequence Data Restriction Mapping Cellulase Exon shuffling Protein Structure Secondary Primer extension Exon Genetics Coding region Amino Acid Sequence Cloning Molecular DNA Fungal Gene Base Sequence Molecular Structure Sequence Homology Amino Acid biology Fungal genetics Intron Exons General Medicine Introns biology.protein DNA Probes |
| Zdroj: | Current Genetics. 30:56-61 |
| ISSN: | 1432-0983 0172-8083 |
| Popis: | The cellulase gene cel3 has been isolated from Agaricus bisporus and sequenced. The 5'-end of the cel3 transcript was determined by primer extension and S1 nuclease protection. Putative regulatory elements have been identified in the cel3 promoter and 3'-untranslated regions. The cel3 coding region is interrupted by six short introns, two of which separate the coding regions for the three modules in the CEL3 protein: cellulose-binding domain, linker region, and catalytic domain. Three of the remaining four introns are positioned in regions coding for loops between structural moieties. Intron positions are conserved between cel3 and other related cellulases. |
| Databáze: | OpenAIRE |
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