Cloning and characterization ofifitm1andifitm3expression during early zebrafish development
| Autor: | Meng-Xi Qiu, Huan-Nan Wang, Fengjiao Deng, Wei-Wei Xue, Jiang-Dong Liu, Jing Che, Zhi-Meng Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine Embryo Nonmammalian Epiboly Organogenesis In situ hybridization 03 medical and health sciences Rapid amplification of cDNA ends Testis medicine Animals Cloning Molecular Zebrafish biology Ovary Gene Expression Regulation Developmental Embryo Cell Biology Zebrafish Proteins biology.organism_classification Antigens Differentiation Cell biology Reverse transcription polymerase chain reaction 030104 developmental biology medicine.anatomical_structure Female Germ cell Developmental Biology |
| Zdroj: | Zygote. 24:149-158 |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199414000756 |
| Popis: | SummaryThe family of interferon-inducible transmembrane proteins (IFITMs) plays a crucial role in inhibiting proliferation, promoting homotypic cell adhesion and mediating germ cell development. In the present study, the full-length cDNAs of zebrafishifitm1(744 bp) andifitm3(702 bp) were obtained by rapid amplification of cDNA ends (RACE). Reverse transcription polymerase chain reaction (RT-PCR) analysis showed thatifitm1mRNA was expressed in the ovary, testis, brain, muscle, liver and kidney, whileifitm3mRNA was only detected in the ovary. Based onin situhybridization,ifitm1mRNA was found to be strongly expressed in the ooplasm from stage I to stage II andifitm3mRNA was also strongly expressed in the ooplasm from stage I to stage II, furthermoreifitm3expression ultimately localized to the cortex region beneath the plasma membrane of stage IV oocytes. During development,ifitm1expression was initially detected in the enveloping layer cells and deep layer cells of shield stage embryos. Then, throughout the segmentation phase (10.25–24 hours post-fertilization (hpf)),ifitm1expression was mainly detected in the head, trunk and tail regions. Unlikeifitm1,ifitm3expression was initially detected in sphere stage embryos and was then broadly expressed throughout the embryo from the 70% epiboly stage to 24 hpf. Interestingly,ifitm3was also expressed in primordial germ cells (PGCs) from the bud stage to 24 hpf. This expression analysis indicates that zebrafishifitm1may play a critical role in early organogenesis and may perform immune or hematopoietic functions andifitm3might be necessary for PGC migration and the formation of female germ cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|