Serum platelet-activating factor acetylhydrolase (PAF-AH) activity in more than 3000 healthy Japanese
| Autor: | Koji Mizuno, Tetsuya Kosaka, Masafumi Yamaguchi, Kazuo Miyanaga |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_specialty Apolipoprotein B Clinical Biochemistry Biochemistry Phospholipases A Eating chemistry.chemical_compound Sex Factors Reference Values Internal medicine medicine PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE Humans Circadian rhythm Triglycerides biology Platelet-activating factor Diet study Chemistry Biochemistry (medical) Mean value Age Factors General Medicine Middle Aged 1-Alkyl-2-acetylglycerophosphocholine Esterase medicine.disease Circadian Rhythm Diet Menopause Cholesterol Endocrinology Spectrophotometry biology.protein Female lipids (amino acids peptides and proteins) |
| Zdroj: | Clinica Chimica Acta. 312:179-183 |
| ISSN: | 0009-8981 |
| Popis: | Background : A spectrophotometric assay for platelet-activating factor acetylhydrolase (PAF-AH) activity differs from the radioisotopic assay in its value because of a difference in substrate specificity. The spectrophotometric assay is more precise than the radioisotopic assay, providing information that is not clear with the radioisotopic assay. Methods : We measured the serum PAF-AH activity in 3106 healthy Japanese, utilizing the spectrophotometric assay with an Hitachi 7170 automatic analyzer. We also measured the serum PAF-AH activity in 18 healthy volunteers to investigate the effect of diet and the change in activity in a day and over 6 weeks. Changes were examined at 0 (day 1), 1, 2, 4 and 6 weeks. Results : The mean value for females was significantly lower than that of males at the 5% level and both male and female activity had a tendency to increase with advancing age. It is known that the PAF-AH is primarily associated with LDL in blood and the PAF-AH activity correlated with the total cholesterol ( r =0.52, n =126) and the LDL cholesterol ( r =0.60, n =126) concentrations. In the diet study, there was no observable effect on activity. No difference in PAF-AH activity was observed between serum and plasma sample types. The serum PAF-AH activity was stable at 7 °C for at least 7 days and at −20 °C for at least 2 months. Conclusions : The serum PAF-AH activity in women was lower than in men until the menopausal age was reached. We could use not only fresh fasting serum, but also plasma sample, non-fasting sample and stored sample to estimate the PAF-AH activity. |
| Databáze: | OpenAIRE |
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