Osteopontin Gene Polymorphisms rs1126616 CT and rs1126772 AG are Associated with Atopic Dermatitis in Polish Population
Autor: | Mieszko Lachota, Anna Woźniacka, Beata Kaleta, Jacek Łukaszkiewicz, Jarosław Bogaczewicz |
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Rok vydání: | 2021 |
Předmět: |
OPN
medicine.medical_specialty osteopontin Single-nucleotide polymorphism Gastroenterology polymorphism Pathogenesis Polymorphism (computer science) Internal medicine Genotype Genetics medicine Allele gene Genetics (clinical) Original Research atopic dermatitis business.industry Haplotype AD Atopic dermatitis asthma medicine.disease The Application of Clinical Genetics Biomarker (medicine) business |
Zdroj: | The Application of Clinical Genetics |
ISSN: | 1178-704X |
Popis: | Beata Kaleta,1 Mieszko Lachota,1,2 Jacek Łukaszkiewicz,3 Anna Woźniacka,4 Jarosław Bogaczewicz5 1Department of Clinical Immunology, Medical University of Warsaw, Warsaw, Poland; 2Doctoral School, Medical University of Warsaw, Warsaw, Poland; 3Department of Biochemistry and Clinical Chemistry, Medical University of Warsaw, Warsaw, Poland; 4Department of Dermatology and Venereology, Medical University of Lodz, Lodz, Poland; 5University of Economics and Human Sciences in Warsaw, Warsaw, PolandCorrespondence: Beata KaletaDepartment of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59 Street, Warsaw, 02-006, PolandTel +48 600 301 690Fax +48 502 21 59Email beata.kaleta@wum.edu.plPurpose: Atopic dermatitis (AD) is a chronic, relapsing inflammatory disease, caused by environmental and genetic factors, which lead to immunological abnormalities. Osteopontin (OPN), also named secreted phosphoprotein 1 (SPP1), is a protein involved in the pathogenesis of numerous autoimmune and inflammatory conditions. However, its role in AD has not been fully elucidated. Therefore, we aim to gain an insight into the role of OPN in AD pathogenesis through investigating its gene single nucleotide polymorphisms (SNPs) and their possible associations with disease clinical features.Patients and Methods: A total of 182 Caucasian participants (45 AD patients and 137 gender- and age-matched controls) were studied. Genomic DNA was isolated from peripheral blood samples. Genotyping for the rs1126616 C>T, rs1126772 A>G, rs9138 A>C, and rs3841116 T>G SNPs was performed by real time polymerase chain reaction (RT-PCR).Results: The frequency of the minor TT genotype and the T allele of rs1126616 C>T was higher in AD patients compared to controls (P = 0.019, OD = 4.86, 95% CI = 1.46â 16.20, and P = 0.047, OR = 1.77, 95% CI = 1.04â 3.00, respectively) and was associated with the higher prevalence of asthma (P = 0.017, OR = 3.73, 95% CI = 0.71â 19.67, and P = 0.004, OR = 3.96, 95% CI = 1.53â 10.25, respectively). Likewise, the minor GG genotype and the G allele of rs1126772 A>G were more frequent in AD patients (P = 0.026, OR = 3.27, 95% CI = 1.29â 8.33, and P = 0.013, OR = 1.94, 95% CI = 1.18â 3.21, respectively) and were associated with the increased incidence of asthma (P = 0.016, OR = 5.06, 95% CI = 1.14â 22.49, and P = 0.002, OR = 4.40, 95% CI = 1.71â 11.35, respectively). Furthermore, haplotype frequency estimation determined the four-loci haplotype TGCT, as a significant risk factor for AD compared to controls (P = 0.031, OR = 9.48, 95% CI = 1.23â 71.91).Conclusion: Our results suggest that the variation in the OPN gene might be associated with AD and increased incidence of asthma in Caucasians. Further studies should be conducted to look into the possible role of OPN as a biomarker for AD.Keywords: asthma, atopic dermatitis, gene, osteopontin, polymorphism, AD, OPN |
Databáze: | OpenAIRE |
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