SHCBP1 is a novel target and exhibits tumor‑promoting effects in gastric cancer
| Autor: | Haibo Yu, Guang‑Jin Tian, Ya Dong Dong, Yan Li Yuan, Deyu Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research proliferation Cell Down-Regulation Apoptosis SHCBP1 03 medical and health sciences 0302 clinical medicine Cyclin D1 Cell Movement Stomach Neoplasms Cell Line Tumor Biomarkers Tumor medicine Humans metastasis RNA Small Interfering Caspase Cell Proliferation Oncogene biology Caspase 3 Chemistry Cell growth gastric cancer Cell Cycle Stomach Cyclin-Dependent Kinase 4 Articles General Medicine Cell cycle Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Shc Signaling Adaptor Proteins Oncology Gene Knockdown Techniques 030220 oncology & carcinogenesis biology.protein Cancer research Signal transduction Signal Transduction |
| Zdroj: | Oncology Reports |
| ISSN: | 1791-2431 1021-335X |
| Popis: | The present study investigated the expression and potential influence of SHC SH2 domain-binding protein 1 (SHCBP1) in gastric cancer (GC) cells. SHCBP1 is closely related to cell proliferation and cell cycle progression, but its role in GC remains unclear. The TCGA database revealed that SHCBP1 is highly expressed in GC tissues. Furthermore, SHCBP1 was revealed to be highly expressed in GC cell lines MGC-803 and SGC-7901 cells, and downregulation of SHCBP1 significantly inhibited GC cell proliferation. Furthermore, SHCBP1 expression promoted cell cycle progression and inhibition of apoptosis. Since the CDK4, cyclin D1 and caspase family proteins play important roles in cell cycle and apoptosis regulation, it was examined whether there was an association between SHCBP1 and these signaling pathways in GC. Our results revealed that SHCBP1 promoted cell cycle progression by regulating the CDK4-cyclin D1 cascade and suppressed caspase-3, caspase PARP-dependent apoptotic pathways. Cell invasion and metastasis experiments also revealed that SHCBP1 promoted tumor growth and invasiveness. These tumor-promoting functions of SHCBP1 may provide a potential molecular basis for the diagnosis and targeted therapy of GC. |
| Databáze: | OpenAIRE |
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