Flavonoids from Potentilla parvifolia Fisch. and Their Neuroprotective Effects in Human Neuroblastoma SH-SY5Y Cells in vitro
| Autor: | Xue-yan Hao, Zhenhua Wang, Guangxiang Luan, Zhenzhen Yuan, Gang Li, Yourui Suo, Honglun Wang, Ji Li |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
SH-SY5Y Ayanin Bioengineering tau Proteins Biochemistry 03 medical and health sciences chemistry.chemical_compound Neuroblastoma 0302 clinical medicine Western blot Alzheimer Disease Cell Line Tumor medicine Humans Phosphorylation Molecular Biology Miquelianin Flavonoids medicine.diagnostic_test Plant Extracts Cell Differentiation General Chemistry General Medicine Okadaic acid 030104 developmental biology Neuroprotective Agents chemistry 030220 oncology & carcinogenesis Potentilla Molecular Medicine Tricin Astragalin Quercetin |
| Zdroj: | Chemistrybiodiversity. 14(6) |
| ISSN: | 1612-1880 |
| Popis: | Potentilla parvifolia Fisch. (Rosaceae) is a traditional medicinal plant in P. R. China. In this study, seven flavonoids, ayanin (1), tricin (2), quercetin (3), tiliroside (4), miquelianin (5), isoquercitrin (6), and astragalin (7), were separated and purified from ethyl acetate extractive fractions from ethanol extracts of P. parvifolia using a combination of sevaral chromatographic methods. The human neuroblastoma SH-SY5Y cells were differentiated with all trans-retinoic acid and treated with okadaic acid to induce tau protein phosphorylation and synaptic atrophy, which could establish an Alzheimer's disease cell model. The neuroprotective effects of these flavonoids in cellular were evaluated in vitro by this cell model. Results from the Western blot and morphology analysis suggested that compounds 3 and 4 had the better neuroprotective effects. |
| Databáze: | OpenAIRE |
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