Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli
| Autor: | Thomas W. North, Yaqi Zhu, J. A. Griffin, G. L. Hansen, Cheng-Kon Shih |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Feline immunodeficiency virus
animal diseases viruses Molecular Sequence Data Immunodeficiency Virus Feline Biology medicine.disease_cause Virus Escherichia coli medicine Pharmacology (medical) Amino Acid Sequence Cloning Molecular Pharmacology Base Sequence Dideoxynucleosides Virion Nucleic acid sequence virus diseases RNA-Directed DNA Polymerase biochemical phenomena metabolism and nutrition biology.organism_classification Nucleotidyltransferase Virology Molecular biology Enterobacteriaceae Recombinant Proteins Reverse transcriptase Infectious Diseases DNA Viral Research Article |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 38:388-391 |
| ISSN: | 1098-6596 0066-4804 |
| Popis: | Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|