Pretreatment with Yeast-Derived Complex Dietary Polysaccharides Suppresses Gut Inflammation, Alters the Microbiota Composition, and Increases Immune Regulatory Short-Chain Fatty Acid Production in C57BL/6 Mice
Autor: | Radhika Gudi, Jada Suber, Robert S. Brown, Chenthamarakshan Vasu, Benjamin M. Johnson |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Male medicine.medical_specialty beta-Glucans medicine.drug_class medicine.medical_treatment Antibiotics Green Fluorescent Proteins Medicine (miscellaneous) Saccharomyces cerevisiae Gut flora 03 medical and health sciences Feces Mice 0302 clinical medicine Immune system Polysaccharides Internal medicine medicine Animals Gene Knock-In Techniques Colitis Nutrition and Dietetics biology Short-chain fatty acid Dextran Sulfate Immunity Forkhead Transcription Factors Neomycin Nutritional Immunology biology.organism_classification medicine.disease Fatty Acids Volatile Gastrointestinal Microbiome Intestines Mice Inbred C57BL 030104 developmental biology Endocrinology Cytokine 030220 oncology & carcinogenesis Female medicine.drug |
Zdroj: | J Nutr |
ISSN: | 1541-6100 |
Popis: | BACKGROUND: β-Glucans (BGs), a group of complex dietary polysaccharides (CDPs), are available as dietary supplements. However, the effects of orally administered highly purified BGs on gut inflammation are largely unknown. OBJECTIVES: The aim of this study was to investigate the impact of orally administering highly purified, yeast-derived BG (YBG; β-1,3/1,6-d-glucan) on susceptibility to colitis. METHODS: Eight-week-old C57BL/6 (B6) mice were used in a series of experiments. Experiment (Expt) 1: male and female mice were treated every day, for 40 d, with saline (control) or 250 μg YBG, followed by 2.5% (wt:vol) dextran sulfate sodium (DSS) in drinking water during days 30–35; and colitis severity and intestinal immune phenotype were determined. Expt 2: female B6 mice were treated with saline or YBG for 30 d and intestinal immune phenotype, gut microbiota composition, and fecal SCFA concentrations were determined. Expt 3: female B6 mice were treated as in Expt 2, given drinking water with or without antibiotics [Abx; ampicillin (1 g/L), vancomycin (0.5 g/L), neomycin (1 g/L), and metronidazole (1 g/L)] during days 16–30, and gut immune phenotype and fecal SCFA concentrations were determined. Expt 4: female B6 Foxp3–green fluorescent protein (-GFP) reporter mice were treated as in Expt 3, and intestinal T-regulatory cell (Treg) frequencies and immune phenotypes were determined. Expt 5: female mice were treated as in Expt 1, given drinking water with or without antibiotics during days 16–40, and colitis severity and intestinal cytokine production were determined. RESULTS: Compared with controls, the YBG group in Expt 1 exhibited suppressive effects on features of colitis, such as loss of body weight (by 47%; P 100%; P |
Databáze: | OpenAIRE |
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