Identification of morphine in the adrenal medullary chromaffin PC-12 cell line
| Autor: | Maria Bougaeva, Benjamin S. Weeks, George B. Stefano, Patrick Cadet, Federico Casares, Yannick Goumon, Bruce J. Brownawell, Wei Zhu |
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| Rok vydání: | 2000 |
| Předmět: |
endocrine system
medicine.medical_specialty Transcription Genetic Adrenal Gland Neoplasms Receptors Opioid mu Pheochromocytoma Biology PC12 Cells Cellular and Molecular Neuroscience Internal medicine medicine Animals Humans Receptor Molecular Biology Chromatography High Pressure Liquid Morphine Reverse Transcriptase Polymerase Chain Reaction Adrenal gland Rats medicine.anatomical_structure Endocrinology Adrenal Medulla Cell culture Chromaffin cell Opiate Adrenal medulla Endocrine gland medicine.drug |
| Zdroj: | Molecular Brain Research. 81:177-180 |
| ISSN: | 0169-328X |
| Popis: | Morphine was identified in the adrenal medulla chromaffin PC-12 cell line by reversed-phase HPLC, following liquid and solid extraction. The morphine corresponding HPLC fractions (1.746+/-0.615 ng of morphine/million cells) were further analyzed by gas chromatography-mass spectrometry and found to be identical to synthetic morphine. Furthermore, using primers derived from the human neuronal mu 1 opiate receptor, we used RT-PCR to detect expression of mu transcripts from this cell line. The transcript was absent. The study conclusively proves morphine, but not a mu opiate receptor, is constitutively expressed in the adrenal medulla chromaffin PC-12 cell line. |
| Databáze: | OpenAIRE |
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