A Comparative Study of Receptor-Targeted Magnetosome and HSA-Coated Iron Oxide Nanoparticles as MRI Contrast-Enhancing Agent in Animal Cancer Model

Autor: Laura E. Ruff, Damla Bozkurt, Öznur Akbal, Murat Demirbilek, Yasan Yeh, Yasin Senel, Berrak Gumuskaya, Şeyda Çolak, Ahmet Çabuk, Emir Baki Denkbaş, Oktay Algin, Sadik C. Esener, Ebru Erdal
Přispěvatelé: [Erdal, Ebru] Aksaray Univ, Dept Biol, Aksaray, Turkey -- [Demirbilek, Murat] Hacettepe Univ, Adv Technol Applicat & Res Ctr, Ankara, Turkey -- [Yeh, Yasan] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA -- [Akbal, Oznur] Gazi Univ, Polatli Art & Sci Fac, Dept Biol, Ankara, Turkey -- [Ruff, Laura -- Esener, Sadik] Univ Calif San Diego, Moores Canc Ctr, La Jolla, CA 92093 USA -- [Bozkurt, Damla -- Cabuk, Ahmet] Osmangazi Univ, Dept Biol, Eskisehir, Turkey -- [Cabuk, Ahmet] Osmangazi Univ, Biotechnol & Biosafety Div Dept, Eskisehir, Turkey -- [Senel, Yasin] Ankara Univ, Vet Internal Med Div, Ankara, Turkey -- [Gumuskaya, Berrak] Yildirim Beyazit Univ, Ataturk Educ & Res Hosp, Dept Patol, Ankara, Turkey -- [Algin, Oktay] Yildirim Beyazit Univ, Ataturk Educ & Res Hosp, Dept Radiol, Ankara, Turkey -- [Colak, Seyda] Hacettepe Univ, Dept Engn Phys, TR-06800 Ankara, Turkey -- [Esener, Sadik] Univ Calif San Diego, Dept Elect & Comp Engn, La Jolla, CA 92093 USA -- [Denkbas, Emir B.] Hacettepe Univ, Div Biochem, Dept Chem, Ankara, Turkey, Gumuskaya, Berrak -- 0000-0003-0599-8968, Sabire Yazıcı Fen-Edebiyat Fakültesi
Rok vydání: 2017
Předmět:
Zdroj: Applied Biochemistry and Biotechnology. 185:91-113
ISSN: 1559-0291
0273-2289
DOI: 10.1007/s12010-017-2642-x
Popis: WOS: 000431499100008
PubMed: 29082480
Magnetosomes are specialized organelles arranged in intracellular chains in magnetotactic bacteria. The superparamagnetic property of these magnetite crystals provides potential applications as contrast-enhancing agents for magnetic resonance imaging. In this study, we compared two different nanoparticles that are bacterial magnetosome and HSA-coated iron oxide nanoparticles for targeting breast cancer. Both magnetosomes and HSA-coated iron oxide nanoparticles were chemically conjugated to fluorescent-labeled anti-EGFR antibodies. Antibody-conjugated nanoparticles were able to bind the MDA-MB-231 cell line, as assessed by flow cytometry. To compare the cytotoxic effect of nanoparticles, MTT assay was used, and according to the results, HSA-coated iron oxide nanoparticles were less cytotoxic to breast cancer cells than magnetosomes. Magnetosomes were bound with higher rate to breast cancer cells than HSA-coated iron oxide nanoparticles. While 250 mu g/ml of magnetosomes was bound 92 +/- 0.2%, 250 mu g/ml of HSA-coated iron oxide nanoparticles was bound with a rate of 65 +/- 5%. In vivo efficiencies of these nanoparticles on breast cancer generated in nude mice were assessed by MRI imaging. Anti-EGFR-modified nanoparticles provide higher resolution images than unmodified nanoparticles. Also, magnetosome with anti-EGFR produced darker image of the tumor tissue in T2-weighted MRI than HSA-coated iron oxide nanoparticles with anti-EGFR. In vivo MR imaging in a mouse breast cancer model shows effective intratumoral distribution of both nanoparticles in the tumor tissue. However, magnetosome demonstrated higher distribution than HSA-coated iron oxide nanoparticles according to fluorescence microscopy evaluation. According to the results of in vitro and in vivo study results, magnetosomes are promising for targeting and therapy applications of the breast cancer cells.
Hacettepe University [014BIYB604002]
This study was supported by Hacettepe University. Project number is 014BIYB604002.
Databáze: OpenAIRE
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