Comparison of Automated and Manual DNA Isolation Methods of Liquid-Based Cytology Samples
| Autor: | María Lourdes Garza-Rodríguez, Antonio Alí Pérez-Maya, Oscar Vidal-Gutiérrez, Víctor H. Barajas-Olmos, Gerardo I Magallanes-Garza, Servando Cardona-Huerta, Irma G. Dominguez-Vigil, Daniel Humberto Méndez-Lozano, Lenny Gallardo-Alvarado, Hugo A. Barrera-Saldaña, David Cantu-de Leon |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Oncology medicine.medical_specialty cervical cancer Uterine Cervical Neoplasms Medicine (miscellaneous) General Biochemistry Genetics and Molecular Biology Specimen Handling Young Adult 03 medical and health sciences 0302 clinical medicine Internal medicine Cytology medicine Humans liquid-based cytology quality control Early Detection of Cancer Aged Biological Specimen Banks Aged 80 and over Cervical cancer 030219 obstetrics & reproductive medicine business.industry Liquid Biopsy 0402 animal and dairy science DNA Neoplasm Original Articles 04 agricultural and veterinary sciences Cell Biology General Medicine medicine.disease 040201 dairy & animal science Biobank DNA extraction biobank nucleic acids Liquid-based cytology biomarker Biomarker (medicine) Female business |
| Zdroj: | Biopreservation and Biobanking |
| ISSN: | 1947-5543 1947-5535 |
| DOI: | 10.1089/bio.2018.0148 |
| Popis: | Liquid-based cytology (LBC) has been used as a diagnostic tool for cervical cancer for years and is now being adopted for other gynecological cancers. LBC represents an important challenge to ensure that the process yields representative biospecimens for quality control (QC) of diagnostic procedures. In this study, we compare QC parameters (integrity, yield and purity, and polymerase chain reaction [PCR] amplification) of DNA isolated from LBC (N = 296) using two different nucleic acid isolation methods, manual (n = 233) or automated (n = 63). We also evaluated two different types of cytological brushes for sampling from the cervix. Our results suggest that manual isolation (yield 22.81 ± 1.92 μg) resulted in increased DNA recovery when compared with automated isolation (yield 9.96 ± 1.11 μg) from LBC samples, with a p-value of |
| Databáze: | OpenAIRE |
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