Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential

Autor: Narihito Nagoshi, Taro Matsumoto, Rina Fujii-Tezuka, Ichiro Morioka, Hideyuki Okano, Shori Takahashi, Hideo Mugishima, Mika Ishige-Wada
Rok vydání: 2021
Předmět:
0301 basic medicine
Medicine (General)
DMEM
Dulbecco's modified Eagle medium
MSCs
mesenchymal stem cells
WJ
Wharton's jelly
FGF-2
fibroblast growth factor-2
ASMA
α-smooth muscle actin
vWF
von Willebrand factor
PDGF
platelet-derived growth factor
0302 clinical medicine
Immunophenotyping
βME
β-mercaptoethanol
DAPI
4′
6-diamino-2-phenylindole
p75 neurotrophin receptor
UA
umbilical artery
CFU-F
colony-forming unit fibroblast
FSK
forskolin
EdU
5-ethynyl-2′-deoxyuridine
UC
umbilical cord
Neural crest
EGFP
enhanced green fluorescent protein
Cell biology
medicine.anatomical_structure
NCSCs
neural crest-derived stem cells
CD146
Original Article
Pericyte
Neural crest stem cells
Stem cell
α-MEM
alpha-modified minimum essential medium
BDNF
bone-derived neurotrophic factor
PBS
phosphate-buffered saline
Biomedical Engineering
Neurosphere
Biology
p75NTR
p75 neurotrophin receptor
Biomaterials
MAP2
microtubule-associated protein 2
03 medical and health sciences
R5-920
FBS
fetal bovine serum
medicine
CD90
NG2
neuron-glial antigen 2
Umbilical cord
EGF
epidermal growth factor
QH573-671
Mesenchymal stem cell
GFAP
glial fibrillary acidic protein
NF200
neurofilament 200
TBS
Tris-buffered saline
030104 developmental biology
UV
umbilical vein
Mesenchymal stem cells
sense organs
Cytology
RA
all-trans-retinoic acid
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Regenerative Therapy
Regenerative Therapy, Vol 16, Iss, Pp 1-11 (2021)
ISSN: 2352-3204
DOI: 10.1016/j.reth.2020.12.002
Popis: Introduction The p75 neurotrophin receptor (p75NTR) is known as an efficient marker for the prospective isolation of mesenchymal stem cells (MSCs) and neural crest-derived stem cells (NCSCs). To date, there is quite limited information concerning p75NTR-expressing cells in umbilical cord (UC), although UC is known as a rich source of MSCs. We show for the first time the localization, phenotype, and functional properties of p75NTR+ cells in UC. Methods Human UC tissue sections were subjected to immunohistochemistry for MSC markers including p75NTR. Enzymatically isolated umbilical artery (UA) cells containing p75NTR+ cells were assessed for immunophenotype, clonogenic capacity, and differentiation potential. To identify the presence of neural crest-derived cells in the UA, P0-Cre/Floxed-EGFP reporter mouse embryos were used, and immunohistochemical analysis of UC tissue was performed. Results Immunohistochemical analysis revealed that p75NTR+ cells were specifically localized to the subendothelial area of the UA and umbilical vein. The p75NTR+ cells co-expressed PDGFRβ, CD90, CD146, and NG2, phenotypic markers of MSCs and pericytes. Isolated UA cells possessed the potential to form neurospheres that further differentiated into neuronal and glial cell lineages. Genetic lineage tracing analysis showed that EGFP+ neural crest-derived cells were detected in the subendothelial area of UA with p75NTR immunoreactivity. Conclusions These results show that UA tissue harbors p75NTR+ pericyte-like cells in the subendothelial area that have the capacity to form neurospheres and the potential for neurogenic differentiation. The lineage tracing data suggests the p75NTR+ cells are putatively derived from the neural crest.
Databáze: OpenAIRE
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