Transcription-coupled deposition of histone modifications during MHC class II gene activation
| Autor: | Walter Reith, Elisa Leimgruber, Natalia Rybtsova, Queralt Seguín-Estévez, Michal Krawczyk, Isabelle Dunand-Sauthier |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Transcriptional Activation
5' Flanking Region Genes MHC Class II HLA-DR alpha-Chains Biology ddc:616.07 Methylation Cell Line Histone H4 Histones Histone H3 Interferon-gamma Histone H1 Histone methylation Histone H2A Genetics Histone code Humans Histone octamer Molecular Biology HLA-DR Antigens/biosynthesis/ genetics Acetylation Interferon-gamma/pharmacology HLA-DR Antigens Molecular biology Kinetics Histone methyltransferase Histones/ metabolism Protein Processing Post-Translational |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Vol. 35, No 10 (2007) pp. 3431-3441 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Posttranslational histone modifications associated with actively expressed genes are generally believed to be introduced primarily by histone-modifying enzymes that are recruited by transcription factors or their associated co-activators. We have performed a comprehensive spatial and temporal analyses of the histone modifications that are deposited upon activation of the MHC class II gene HLA-DRA by the co-activator CIITA. We find that transcription-associated histone modifications are introduced during two sequential phases. The first phase precedes transcription initiation and is characterized exclusively by a rapid increase in histone H4 acetylation over a large upstream domain. All other modifications examined, including the acetylation and methylation of several residues in histone H3, are restricted to short regions situated at or within the 5' end of the gene and are established during a second phase that is concomitant with ongoing transcription. This second phase is completely abrogated when elongation by RNA polymerase II is blocked. These results provide strong evidence that transcription elongation can play a decisive role in the deposition of histone modification patterns associated with inducible gene activation. |
| Databáze: | OpenAIRE |
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