An integrated 3D fluidic device with bubble guidance mechanism for long-term primary and secondary cell recordings on multi-electrode array platform
| Autor: | Anil Krishna Konduri, K. S. Narayan, C S Deepak, S Purohit |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Light
Microfluidics 0206 medical engineering Cell Biomedical Engineering Bioengineering Chick Embryo 02 engineering and technology Biochemistry Retinal ganglion Retina Biomaterials medicine Electrode array Animals Fluidics Primary cell Electrodes Chemistry General Medicine 021001 nanoscience & nanotechnology 020601 biomedical engineering Electrophysiological Phenomena Electrophysiology medicine.anatomical_structure 0210 nano-technology Biotechnology Biomedical engineering Explant culture |
| Zdroj: | Biofabrication. 12:045019 |
| ISSN: | 1758-5090 1758-5082 |
| DOI: | 10.1088/1758-5090/aba500 |
| Popis: | A 3D fluidic device (3D-FD) is designed and developed with the capability of auto bubble guidance via a helical pathway in a 3D geometry. This assembly is integrated to a multi-electrode array (MEA) to maintain secondary cell lines, primary cells and primary retinal tissue explants of chick embryos for continuous monitoring of the growth and electrophysiology recording. The ability to maintain the retinal tissue explant, extracted from day 14 (E-14) and day 21 (E-21) chick embryos in an integrated 3D-FD MEA for long duration (>100 h) and study the development is demonstrated. The enhanced duration of monitoring offered by this device is due to the controlled laminar flow and the maintenance of a stable microenvironment. The spontaneous electrical activity of the retina, including the spike recordings from the retinal ganglion layer, was monitored over a long duration. Specifically, the spiking activity in embryonic chick retinas of different days (E-14 to 21) is studied, and the presence of light-stimulated firings along with a distinct electroretinogram for E-21 mature retina provides the evidence of a stable microenvironment over a sustained period. |
| Databáze: | OpenAIRE |
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