A comparative study on potential cytogenetic fingerprints for radiation LET in human lymphocytes
| Autor: | K. L. Gale, Wen Deng, J. N. Lucas, D. P. Morrison |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Linear energy transfer Chromosomal translocation Biology Chromosomes Translocation Genetic Ionizing radiation Chromosome Painting Cytogenetics medicine Humans Radiology Nuclear Medicine and imaging Linear Energy Transfer Irradiation Lymphocytes Metaphase In Situ Hybridization Fluorescence Genetics Chromosome Aberrations Neutrons Radiological and Ultrasound Technology medicine.diagnostic_test Chromosome Telomere Molecular biology DNA Fingerprinting Beta Particles Genetic Techniques Gamma Rays Fluorescence in situ hybridization |
| Zdroj: | International journal of radiation biology. 76(12) |
| ISSN: | 0955-3002 |
| Popis: | To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes.Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co gamma-rays, 0.9 Gy 3H beta-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by combined FISH with pan-telomere staining and specific whole-chromosome painting (1, 2 and 4). Total chromosome translocations and insertions were also analysed by multicolour whole-chromosome painting (chromosomes 1, 2 and 4 orange, chromosomes 3, 5 and 6 green).Among the six proposed radiation cytogenetic fingerprints, the ratio of total simple translocations to insertions (I-ratio), showed the largest difference between low-LET 60Co gamma-ray and high-LET neutron radiation. The ratios of complete exchanges to incomplete rejoinings [S(I)-ratio] and dicentrics to interstitial deletions (H-ratio), showed a similar significant difference between low- and high-LET radiation. The ratios of centric rings to interstitial deletion (G-ratio) showed a trend of LET-related difference, but the difference was not significant in this data set. The ratios of dicentrics to centric rings (F-ratio) and apparent complete exchanges to hidden complete exchanges [S(II)-ratio], showed no difference between low- and high-LET radiation. In the 1426 radiation-induced chromosome aberrations observed after 52 h culture, evidence for sister-chromatid fusion but not telomere addition was found.Pan-telomere staining plus specific whole chromosome painting allows simultaneous and objective detection of complete or incomplete chromosome exchanges and interstitial or terminal deletions in human peripheral lymphocytes. Of the six proposed cytogenetic ratios, the I-ratio is the most effective cytogenetic fingerprint for distinguishing low-LET from high-LET radiation in human metaphase human lymphocytes. |
| Databáze: | OpenAIRE |
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