Genetic and epigenetic status of triple exotic consanguinity cotton introgression lines
| Autor: | S.P. He, J.L. Sun, X.M. Du |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Genetics
Germplasm Epigenomics Amplified Fragment Length Polymorphism Analysis Gossypium Polymorphism Genetic DNA Plant Introgression Genetic Variation General Medicine Biology DNA Methylation Polymorphism (computer science) Genetic variation DNA methylation Amplified fragment length polymorphism Inbreeding Molecular Biology |
| Zdroj: | Genetics and molecular research : GMR. 10(4) |
| ISSN: | 1676-5680 |
| Popis: | Introgression lines are some of the most important germplasm for breeding applications and other research conducted on cotton crops. The DNA methylation level among 10 introgression lines of cotton (Gossypium hirsutum) and three exotic parental species (G. arboreum, G. thurberi and G. barbadense) were assessed by methylation-sensitive amplified polymorphism (MSAP) technology. The methylation level in the introgression lines ranged from 33.3 to 51.5%. However, the lines PD0111 and PD0113 had the lowest methylation level (34.6 and 33.3%, respectively) due to demethylation of most non-coding sequences. Amplified fragment length polymorphism (AFLP) was used to evaluate the genetic polymorphism in the cotton introgression lines. A high degree of polymorphism was observed in all introgression lines (mean 47.2%) based on AFLP and MSAP analyses. This confirmed the effects of genetic improvement on cotton introgression lines. The low methylation varieties, PD0111 and PD0113 (introgression lines), clustered outside of the introgression lines based on MSAP data, which was incongruent with an AFLP-based dendrogram. This phenomenon could be caused by environmental changes or introgression of exotic DNA fragments. |
| Databáze: | OpenAIRE |
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