Genetic characterization of Bagaza virus (BAGV) isolated in India and evidence of anti-BAGV antibodies in sera collected from encephalitis patients
| Autor: | Milind M. Gore, Vijay M. Ayachit, Prasanna N. Yergolkar, Vasudha Sankararaman, Vijay P. Bondre, Pradip V. Fulmali, Akhilesh C. Mishra, Gajanan N. Sapkal |
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| Rok vydání: | 2009 |
| Předmět: |
viruses
Molecular Sequence Data Population India Sequence Homology Biology Antibodies Viral Arbovirus Virus Disease Outbreaks Flavivirus Infections Mice Virology medicine Animals Cluster Analysis Humans Encephalitis Viral education Phylogeny Encephalitis Virus Japanese education.field_of_study Flavivirus virus diseases Outbreak Sequence Analysis DNA Japanese encephalitis medicine.disease Complement fixation test biology.organism_classification Culex tritaeniorhynchus Culex West Nile virus Encephalitis |
| Zdroj: | Journal of General Virology. 90:2644-2649 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | During investigations into the outbreak of encephalitis in 1996 in the Kerala state in India, an arbovirus was isolated from a Culex tritaeniorhynchus mosquito pool. It was characterized as a Japanese encephalitis and West Nile virus cross-reactive arbovirus by complement fixation test. A plaque reduction–neutralization test was performed using hyperimmune sera raised against the plaque-purified arbovirus isolate. The sera did not show reactivity with Japanese encephalitis virus and were weakly reactive with West Nile virus. Complete open reading frame sequence analysis characterized the arbovirus as Bagaza virus (BAGV), with 94.80 % nucleotide identity with African BAGV strain DakAr B209. Sera collected from the encephalitic patients during the acute phase of illness showed 15 % (8/53) positivity for anti-BAGV neutralizing antibodies. This is the first report of the isolation of BAGV from India. The presence of anti-BAGV neutralizing antibodies suggests that the human population has been exposed to BAGV. |
| Databáze: | OpenAIRE |
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