Endoreduplication and polyploidy in fragile X cells induced by methotrexate and fluorodeoxyuridine: implications for diagnosis
| Autor: | M. Werner, Tamar Schaap, Batsheva Kerem, Ruth Goitein, B. Meidan, Chava Kimchi-Sarfaty |
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| Rok vydání: | 1991 |
| Předmět: |
Mitosis
Biology S Phase Polyploidy Gene duplication medicine Amniocyte Endoreduplication Humans Lymphocytes Genetics (clinical) Cells Cultured Lymphoblast Chromosomal fragile site Embryo medicine.disease Amniotic Fluid Embryo Mammalian Hematopoietic Stem Cells Molecular biology Fragile X syndrome Methotrexate Cell culture Fragile X Syndrome Floxuridine Cell Division |
| Zdroj: | American journal of medical genetics. 38(2-3) |
| ISSN: | 0148-7299 |
| Popis: | Lymphoblastoid cell lines from fragile X patients and amniotic cells from fragile X embryos, when cultured with methotrexate (MTX) or fluorodeoxyuridine (FUdR), showed a significant increase in endoreduplication and polyploidy. This phenomenon was not observed in fragile X lymphocytes or in lymphoblastoid cell lines and amniotic cells of normal control individuals. The relationship between the inducible fragile site at Xq27.3 and the inducible endoreduplication is discussed. The induction of endoreduplication and polyploidy in fragile X lymphoblasts and amniocytes is evaluated as a possible diagnostic test. |
| Databáze: | OpenAIRE |
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