Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation
| Autor: | Chen Yi Chen, Hao Yuan Chia, Tz Yu Kuo, Wan Chun Li, Pei Chun Huang, Tsai Ying Chen, Yi Ta Hsieh, Chen Wei Kao, Jyuhn Huarng Juang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine endocrine system Cell Transplantation Swine medicine.medical_treatment Transplantation Heterologous Cell Islets of Langerhans Transplantation Mice Nude lcsh:Medicine Heterologous SOX9 Streptozocin Article Diabetes Mellitus Experimental Andrology Islets of Langerhans Mice 03 medical and health sciences medicine Animals Insulin lcsh:Science Pancreas Cells Cultured Mice Inbred BALB C Budding Multidisciplinary Chemistry lcsh:R Epithelium Transplantation 030104 developmental biology medicine.anatomical_structure Animals Newborn Hyperglycemia PDX1 lcsh:Q |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-26404-6 |
| Popis: | Ductal epithelium is primarily detected in porcine neonatal pancreatic cell clusters (NPCCs) bearing grafts, suggesting that transplants might exhibit progenitor-like phenotypes. Here we found that soon after NPCC isolation, PDX1+/insulin− and SOX9+ pancreatic progenitor-like cells dramatically increased while dual-hormonal progenitor-like cells were routinely observed in NPCC culture. After transplantation (Tx), insulin+ cells increased and PDX1+ and SOX9+ cells gradually decreased in both non-diabetic (NDM) and streptozotocin-induced diabetic (DM) grafts over 2 months. Strikingly, a significantly higher percentage of insulin+ cells were detected in 9-day and 16-day, but not in 23-day, 30-day and 60-day grafts implying that hyperglycemia could only facilitate NPCC-derived β cells early post-Tx. A higher percentage of NPCC-derived β cells in early DM grafts was determined via an enhanced neogenic differentiation based on the detection of insulin+ cells budding out from PDX1+/SOX9+ epithelium. Interestingly, a drop in SOX9+ progenitor-like cells was detected 16 days post-Tx in DM grafts whilst PDX1+ cells do not show a significant difference until 60 days post-Tx between DM and NDM grafts, demonstrating that distinct progenitor-like populations fuel new β cells post-Tx. In conclusion, PDX1+/SOX9+ cells could be quickly activated after NPCC isolation, maintain their multipotency in culture and differentiate into new β cell post-Tx. |
| Databáze: | OpenAIRE |
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