The Effect of Disulfiram in the Prevention of Postoperative Adhesion Formation in an Experimental Rat Uterine Horn Model
Autor: | Özlem Erten, Sadiman Kiykac Altinbas, Omer Lutfi Tapisiz, Gülçin Güler Şimşek, Salim Erkaya, Yıldız Akdaş Reis, Ümit Göktolga |
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Rok vydání: | 2021 |
Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine Infertility medicine.medical_specialty Time Factors VEGF receptors Anti-Inflammatory Agents Administration Oral Adhesion (medicine) Tissue Adhesions Gastroenterology Drug Administration Schedule 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Statistical significance Internal medicine Disulfiram Electrocoagulation medicine Animals Rats Wistar Uterine Diseases 030219 obstetrics & reproductive medicine biology business.industry Uterus Obstetrics and Gynecology Uterine horns Postoperative adhesion medicine.disease Vascular endothelial growth factor Disease Models Animal 030104 developmental biology Matrix Metalloproteinase 9 chemistry biology.protein Matrix Metalloproteinase 2 Female business medicine.drug |
Zdroj: | Reproductive Sciences. 28:2650-2660 |
ISSN: | 1933-7205 1933-7191 |
Popis: | Postoperative adhesions can cause serious complications, including intestinal obstruction, chronic abdominopelvic pain, and infertility in women. Here we investigate the effects of disulfiram on the postoperative adhesion model. Female Wistar rats were used (n = 72). The animals were separated into six groups (12 rats per group): group 1 (control), group 2 (300 mg/kg disulfiram administered for 3 days preoperatively), group 3 (50 mg/kg disulfiram administered for 3 days preoperatively and 14 days postoperatively), group 4 (300 mg/kg disulfiram administered for 3 days preoperatively and 14 days postoperatively), group 5 (50 mg/kg disulfiram administered 14 days postoperatively only), and group 6 (300 mg/kg disulfiram administered 14 days postoperatively only). A histopathologic examination was performed. Immunohistochemical stainings for matrix metalloproteinase-2 and 9 (MMP-2, and MMP-9) and vascular endothelial growth factor (VEGF) were evaluated. The macroscopic adhesion scores were significantly lower in the disulfiram groups (groups 3, 4, and 6) compared to the control group (p < 0.05). Inflammation scores were lower in all groups receiving disulfiram, but only reached statistical significance in group 4 (p < 0.05). In the immunohistochemical evaluation of the groups, MMP-9 was significantly lower in group 5 than group 4 (p < 0.05). There was no significant difference between the groups for MMP-2 and VEGF. We found that disulfiram reduced postoperative adhesion formation. Disulfiram becomes more effective (by directly reducing inflammation) when initiated during the preoperative period at high doses. |
Databáze: | OpenAIRE |
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