The Effect of Disulfiram in the Prevention of Postoperative Adhesion Formation in an Experimental Rat Uterine Horn Model

Autor: Özlem Erten, Sadiman Kiykac Altinbas, Omer Lutfi Tapisiz, Gülçin Güler Şimşek, Salim Erkaya, Yıldız Akdaş Reis, Ümit Göktolga
Rok vydání: 2021
Předmět:
Vascular Endothelial Growth Factor A
0301 basic medicine
Infertility
medicine.medical_specialty
Time Factors
VEGF receptors
Anti-Inflammatory Agents
Administration
Oral
Adhesion (medicine)
Tissue Adhesions
Gastroenterology
Drug Administration Schedule
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Statistical significance
Internal medicine
Disulfiram
Electrocoagulation
medicine
Animals
Rats
Wistar
Uterine Diseases
030219 obstetrics & reproductive medicine
biology
business.industry
Uterus
Obstetrics and Gynecology
Uterine horns
Postoperative adhesion
medicine.disease
Vascular endothelial growth factor
Disease Models
Animal
030104 developmental biology
Matrix Metalloproteinase 9
chemistry
biology.protein
Matrix Metalloproteinase 2
Female
business
medicine.drug
Zdroj: Reproductive Sciences. 28:2650-2660
ISSN: 1933-7205
1933-7191
Popis: Postoperative adhesions can cause serious complications, including intestinal obstruction, chronic abdominopelvic pain, and infertility in women. Here we investigate the effects of disulfiram on the postoperative adhesion model. Female Wistar rats were used (n = 72). The animals were separated into six groups (12 rats per group): group 1 (control), group 2 (300 mg/kg disulfiram administered for 3 days preoperatively), group 3 (50 mg/kg disulfiram administered for 3 days preoperatively and 14 days postoperatively), group 4 (300 mg/kg disulfiram administered for 3 days preoperatively and 14 days postoperatively), group 5 (50 mg/kg disulfiram administered 14 days postoperatively only), and group 6 (300 mg/kg disulfiram administered 14 days postoperatively only). A histopathologic examination was performed. Immunohistochemical stainings for matrix metalloproteinase-2 and 9 (MMP-2, and MMP-9) and vascular endothelial growth factor (VEGF) were evaluated. The macroscopic adhesion scores were significantly lower in the disulfiram groups (groups 3, 4, and 6) compared to the control group (p < 0.05). Inflammation scores were lower in all groups receiving disulfiram, but only reached statistical significance in group 4 (p < 0.05). In the immunohistochemical evaluation of the groups, MMP-9 was significantly lower in group 5 than group 4 (p < 0.05). There was no significant difference between the groups for MMP-2 and VEGF. We found that disulfiram reduced postoperative adhesion formation. Disulfiram becomes more effective (by directly reducing inflammation) when initiated during the preoperative period at high doses.
Databáze: OpenAIRE
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