Characterization of AvaR1, a butenolide-autoregulator receptor for biosynthesis of a Streptomyces hormone in Streptomyces avermitilis
| Autor: | Haruo Ikeda, Hiroyuki Iguchi, Shigeru Kitani, Suandi Pratama Sultan, Takuya Nihira, Kiyoko T. Miyamoto, Tokitaka Atago |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transcription Genetic 030106 microbiology Mutant Receptors Cell Surface Applied Microbiology and Biotechnology Streptomyces Gene Knockout Techniques 03 medical and health sciences chemistry.chemical_compound 4-Butyrolactone Bacterial Proteins Secondary metabolism Avermectin Derepression Butenolide Ivermectin biology Promoter Gene Expression Regulation Bacterial General Medicine biology.organism_classification Repressor Proteins chemistry Biochemistry Streptomyces avermitilis Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 100:9581-9591 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-016-7781-4 |
| Popis: | Streptomyces hormones, sometimes called as autoregulators, are important signaling molecules to trigger secondary metabolism across many Streptomyces species. We recently identified a butenolide-type autoregulator (termed avenolide) as a new class of Streptomyces hormone from Streptomyces avermitilis that produces important anthelmintic agent avermectin. Avenolide triggers the production of avermectin with minimum effective concentration of nanomolar. Here, we describe the characterization of avaR1 encoding an avenolide receptor in the regulation of avermectin production and avenolide biosynthesis. The disruption of avaR1 resulted in transcriptional derepression of avenolide biosynthetic gene with an increase in avenolide production, with no change in the avermectin production profile. Moreover, the avaR1 mutant showed increased transcription of avaR1. Together with clear DNA-binding capacity of AvaR1 toward avaR1 upstream region, it suggests that AvaR1 negatively controls the expression of avaR1 through the direct binding to the promoter region of avaR1. These findings revealed that the avenolide receptor AvaR1 functions as a transcriptional repressor for avenolide biosynthesis and its own synthesis. |
| Databáze: | OpenAIRE |
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