Polymerization Dynamics of the Prophage-Encoded Actin-Like Protein AlpC Is Influenced by the DNA-Binding Adapter AlpA
| Autor: | Andreas Klingl, Catriona Donovan, Nadine Albrecht, Marc Bramkamp, Aaron J. Forde |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) replication lcsh:QR1-502 Biology Corynebacterium DNA segregation Microbiology lcsh:Microbiology DNA sequencing Corynebacterium glutamicum 03 medical and health sciences chemistry.chemical_compound Adapter (genetics) Plasmid AlpA AlpC phage Prophage Actin Original Research DNA transport alpS Molecular biology Cell biology 030104 developmental biology chemistry actin DNA |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 8 (2017) |
| ISSN: | 1664-302X |
| Popis: | The Corynebacterium glutamicum ATCC 13032 prophage CGP3 encodes an actin-like protein, AlpC that was shown to be involved in viral DNA transport and efficient viral DNA replication. AlpC binds to an adapter, AlpA that in turn binds to specific DNA sequences, termed alpS sites. Thus, the AlpAC system is similar to the known plasmid segregation system ParMRS. So far it is unclear how the AlpACS system mediates DNA transport and, whether AlpA and AlpC functionally interact. We show here that AlpA modulates AlpC filamentation dynamics in a dual way. Unbound AlpA stimulates AlpC filament disassembly, while AlpA bound to alpS sites allows for AlpC filament formation. Based on these results we propose a simple search and capture model that explains DNA segregation by viral AlpACS DNA segregation system. |
| Databáze: | OpenAIRE |
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