Transmission, infectivity, and neutralization of a spike L452R SARS-CoV-2 variant

Autor: Carlos Anaya, Jill K. Hacker, Ursula Schulze-Gahmen, Monica Haw, Carl L. Hanson, Chantha Kath, Debra A. Wadford, Miguel Garcia-Knight, Phillip A. Frankino, Mir M. Khalid, Jing Cheng, Claudia Sanchez San Martin, Donna Ferguson, Xianding Deng, Melanie Ott, Steve Miller, Raul Andino, Liana F. Lareau, John Bell, Mary Kate Morris, G. Renuka Kumar, Jennifer M. Hayashi, Charles Y. Chiu, Dustin R. Glasner, Peter V. Lidsky, Peera Hemarajata, Haridha Shivram, Bharath Sreekumar, Stacia K. Wyman, Sarah McMahon, Yinghong Xiao, Nicole M. Green, Scot Federman, Pei-Yi Chen, Venice Servellita, Taha Y. Taha, Nikitha P. Reddy, Jessica Streithorst, Joanna Balcerek, Candace Wang, Jordan E. Taylor, Kevin Reyes, Camille R. Simoneau, Alicia Sotomayor-Gonzalez, Amelia S. Gliwa, Alex Espinosa
Jazyk: angličtina
Rok vydání: 2021
Předmět:
coronavirus
genomic epidemiology
Antibodies
Viral
medicine.disease_cause
spike protein
Medical and Health Sciences
Neutralization
0302 clinical medicine
vaccine
Monoclonal
antibody neutralization
pseudovirus infectivity studies
Viral
Neutralizing
Lung
Infectivity
0303 health sciences
Mutation
biology
L452R mutation
Transmission (medicine)
Antibodies
Monoclonal
Biological Sciences
Spike Glycoprotein
Titer
Infectious Diseases
Spike Glycoprotein
Coronavirus
Pneumonia & Influenza
Antibody
viral whole-genome sequencing
variant of concern
Biotechnology
B.1.427/B.1.429
N501Y mutation
General Biochemistry
Genetics and Molecular Biology
Antibodies
Article
Vaccine Related
03 medical and health sciences
medicine
Humans
Viral shedding
molecular dating
030304 developmental biology
Whole Genome Sequencing
SARS-CoV-2
pandemic
Prevention
COVID-19
Pneumonia
Antibodies
Neutralizing
Virology
genomic surveillance
Coronavirus
Emerging Infectious Diseases
Good Health and Well Being
variant
Cell culture
biology.protein
Immunization
20C/L452R
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Cell
Cell, vol 184, iss 13
medRxiv
article-version (status) pre
article-version (number) 1
ISSN: 1097-4172
0092-8674
Popis: We identified an emerging SARS-CoV-2 variant by viral whole-genome sequencing of 2,172 nasal/nasopharyngeal swab samples from 44 counties in California, a state in the Western United States. Named B.1.427/B.1.429 to denote its 2 lineages, the variant emerged in May 2020 and increased from 0% to >50% of sequenced cases from September 2020 to January 2021, showing 18.6-24% increased transmissibility relative to wild-type circulating strains. The variant carries 3 mutations in the spike protein, including an L452R substitution. We found 2-fold increased B.1.427/B.1.429 viral shedding in vivo and increased L452R pseudovirus infection of cell cultures and lung organoids, albeit decreased relative to pseudoviruses carrying the N501Y mutation common to variants B.1.1.7, B.1.351, and P.1. Antibody neutralization assays revealed 4.0 to 6.7-fold and 2.0-fold decreases in neutralizing titers from convalescent patients and vaccine recipients, respectively. The increased prevalence of a more transmissible variant in California exhibiting decreased antibody neutralization warrants further investigation.
A SARS-CoV-2 variant of concern bearing the L452R spike protein mutation is widely circulating in California, United States, and demonstrates increased transmissibility, infectivity, and avoidance of antibody neutralization.
Databáze: OpenAIRE
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