Effects of hypoxia and nitric oxide on ferritin content of alveolar cells
| Autor: | A. M. Y. R. O'brien-Ladner, Lewis J. Wesselius, Chris R. Kaiser, Jacqueline J. Smith |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
medicine.medical_specialty Iron S-Nitroso-N-Acetylpenicillamine Nitric Oxide Pathology and Forensic Medicine Nitric oxide Cell Line Alveolar cells chemistry.chemical_compound Internal medicine Macrophages Alveolar medicine Tumor Cells Cultured Humans Nitric Oxide Donors A549 cell biology General Medicine respiratory system Cell Hypoxia Ferritin medicine.anatomical_structure Endocrinology chemistry Biochemistry Ferritins Alveolar macrophage biology.protein S-Nitroso-N-acetylpenicillamine Pulmonary alveolus Bronchoalveolar Lavage Fluid Fetal bovine serum |
| Zdroj: | The Journal of laboratory and clinical medicine. 141(5) |
| ISSN: | 0022-2143 |
| Popis: | Concentrations of ferritin in alveolar cells and on the alveolar surface are increased in patients with a variety of respiratory disorders. Ferritin synthesis by cells is modulated by iron content but is also influenced by stimuli other than iron. In this study we sought to determine whether in vitro exposure to hypoxia- or nitric oxide (NO)-induced ferritin accumulation or release by human alveolar macrophages (AMs) or a lung cancer-derived epithelial cell line (A549). Changes in cell content of iron and ferritin (L- and H-types), as well as ferritin content of cell supernatants, were determined after in vitro exposure to hypoxia (1% or 10% O(2), 18 hours) or the NO donor S-nitroso-N-acetylpenicillamine (SNAP, 0.01-1.0 mmol/L, 18 hours). Exposure to 1% O(2) increased ferritin content in both cell types (>fourfold increase; P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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