GABA interaction with lipids in organic medium
| Autor: | Eduardo Lassaga, Dante Beltramo, Augusto Arce, Silvia Kivatinitz |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Ceramide
Glutamic Acid Tritium Binding Competitive General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Glutamates Phosphatidylcholine Amino Acids General Pharmacology Toxicology and Pharmaceutics Phospholipids gamma-Aminobutyric Acid chemistry.chemical_classification Phosphatidylethanolamine Sulfoglycosphingolipids Chemistry Methanol Water General Medicine Glutamic acid Phosphatidic acid Lipid Metabolism Sphingomyelins Amino acid Dissociation constant nervous system Biochemistry Solvents lipids (amino acids peptides and proteins) Chloroform Sphingomyelin |
| Zdroj: | Life Sciences. 41:733-739 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(87)90453-x |
| Popis: | The interaction of 3 H-GABA (gamma-aminobutyric acid and 14 C-glutamate with lipids in an aqueous organic partition system was studied. With this partition system 3 H-GABA and 14 C-glutamate were able to interact with sphingomyelin, sulfatide, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidic acid but not with cholesterol or ceramide. In an homogeneous aqueous medium we could not demonstrate any interaction between 3 H-GABA and lipids. The apparent dissociation constants (K d ) for 3 H-GABA-lipids or 14 C-glutamate-lipids interactions in organic medium were in the millimolar range and maximal charge (B max ) between 3 and 7 moles of GABA or glutamate by mole of lipid. Amino acids such as glutamic acid, β-alanine and glycine displaced 3 H-GABA with the same potency as GABA itself; thus these results show that the interaction lacks pharmacological specificity. To detect this interaction lipid concentrations higher than 2 uM were required and in the partition system 3 H-GABA and lipid phosphorus were both concentrated at the interface. Therefore lipids tested with a biphasic partition system do not fulfill the classical criteria for a neurotransmitter receptor at least not for GABA and glutamate. |
| Databáze: | OpenAIRE |
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